An optimized QF-binary expression system for use in zebrafish.

Animals Animals, Genetically Modified / genetics Gene Expression Neurospora crassa / genetics Protozoan Proteins / genetics Transcription Factors / genetics Zebrafish / genetics
Binary expression system Gal4 Gene tagging Knock-in Lineage tracing Q-system QF QUAS Zebrafish

Journal

Developmental biology
ISSN: 1095-564X
Titre abrégé: Dev Biol
Pays: United States
ID NLM: 0372762

Informations de publication

Date de publication:
15 09 2020
Historique:
received: 21 02 2020
revised: 05 07 2020
accepted: 09 07 2020
pubmed: 23 7 2020
medline: 4 2 2021
entrez: 23 7 2020
Statut: ppublish

Résumé

The zebrafish model organism has been of exceptional utility for the study of vertebrate development and disease through the application of tissue-specific labelling and overexpression of genes carrying patient-derived mutations. However, there remains a need for a binary expression system that is both non-toxic and not silenced over animal generations by DNA methylation. The Q binary expression system derived from the fungus Neurospora crassa is ideal, because the consensus binding site for the QF transcription factor lacks CpG dinucleotides, precluding silencing by CpG-meditated methylation. To optimize this system for zebrafish, we systematically tested several variants of the QF transcription factor: QF full length; QF2, which lacks the middle domain; QF2

Identifiants

DOI: 10.1016/j.ydbio.2020.07.007 PMID: 32697972
pubmed: 32697972
pii: S0012-1606(20)30202-5
doi: 10.1016/j.ydbio.2020.07.007
pii:
doi:

Substances chimiques

Protozoan Proteins 0
Transcription Factors 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

144-156

Subventions

Organisme : CIHR
ID : PJT-159611
Pays : Canada
Organisme : CIHR
ID : OME-142884
Pays : Canada
Organisme : CIHR
ID : GSD-152379
Pays : Canada
Organisme : CIHR
ID : MOP-130294
Pays : Canada
Organisme : CIHR
ID : GSD-140321
Pays : Canada

Informations de copyright

Copyright © 2020 Elsevier Inc. All rights reserved.

Auteurs

Jason Burgess (J)

The Hospital for Sick Children, Program in Developmental and Stem Cell Biology, Toronto, ON, M5G 0A4, Canada.

Jeffrey T Burrows (JT)

The Hospital for Sick Children, Program in Developmental and Stem Cell Biology, Toronto, ON, M5G 0A4, Canada.

Roshan Sadhak (R)

Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.

Sharon Chiang (S)

Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.

Alex Weiss (A)

The Hospital for Sick Children, Program in Developmental and Stem Cell Biology, Toronto, ON, M5G 0A4, Canada.

Cassandra D'Amata (C)

The Hospital for Sick Children, Program in Developmental and Stem Cell Biology, Toronto, ON, M5G 0A4, Canada.

Alyssa M Molinaro (AM)

Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.

Shujun Zhu (S)

The Hospital for Sick Children, Program in Developmental and Stem Cell Biology, Toronto, ON, M5G 0A4, Canada.

Michael Long (M)

The Donnelly Centre for Cellular and Biomolecular Research (CCBR), University of Toronto, Toronto, ON, M5S 3E1, Canada.

Chun Hu (C)

The Donnelly Centre for Cellular and Biomolecular Research (CCBR), University of Toronto, Toronto, ON, M5S 3E1, Canada.

Henry M Krause (HM)

Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada; The Donnelly Centre for Cellular and Biomolecular Research (CCBR), University of Toronto, Toronto, ON, M5S 3E1, Canada.

Bret J Pearson (BJ)

The Hospital for Sick Children, Program in Developmental and Stem Cell Biology, Toronto, ON, M5G 0A4, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada; Ontario Institute for Cancer Research, Toronto, ON, Canada. Electronic address: bret.pearson@sickkids.ca.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › An optimized QF-binary expression system for...
questionsmedicales.fr › Stades d'organismes › Organismes génétiquement modifiés › Animal génétiquement modifié › An optimized QF-binary expression system for...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › An optimized QF-binary expression system for...
questionsmedicales.fr › Eucaryotes › Champignons › Ascomycota › Sordariales › Neurospora › Neurospora crassa › An optimized QF-binary expression system for...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de protozoaire › An optimized QF-binary expression system for...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › An optimized QF-binary expression system for...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Poissons › Cypriniformes › Cyprinidae › Danio zébré › An optimized QF-binary expression system for...