Development of two continuous hemocyte cell sublines in the Asian corn borer Ostrinia furnacalis and the identification of molecular markers for hemocytes.
cell sublines
granulocyte
hemocyte
insect
molecular marker
plasmatocyte
Journal
Insect science
ISSN: 1744-7917
Titre abrégé: Insect Sci
Pays: Australia
ID NLM: 101266965
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
revised:
05
07
2020
received:
05
05
2020
accepted:
08
07
2020
pubmed:
24
7
2020
medline:
30
11
2021
entrez:
24
7
2020
Statut:
ppublish
Résumé
Granulocytes and plasmatocytes play important roles in clearing foreign objects in insects, but it is difficult to distinguish between them in immune reactions. Based on the hemocyte cell line SYSU-OfHe-C established at our lab, two cell sublines, SYSU-OfHe-C Granulocyte (Gr cells) and SYSU-OfHe-C Plasmatocyte (Pl cells), which possess the morphological characteristics of granulocytes and plasmatocytes, respectively, were established. Gr and Pl cells showed different behaviors in immune reactions, such as spreading, phagocytosis and encapsulation. Pl cells were easier to spread, but Gr cells tended to undergo aggregation, indicating that they may take different strategies to clear foreign objects. These results also suggested that granulocytes and plasmatocytes may express some different proteins. By comparing the gene expression in cells from the two sublines, 1662 differentially expressed genes were identified, and 13 out of 30 transmembrane proteins highly expressed in Pl cells (six) or Gr cells (seven) were further screened and confirmed by reverse-transcription polymerase chain reaction (PCR). Finally, three transmembrane genes specifically expressed in Pl cells and two transmembrane genes specifically expressed in Gr cells were screened out based on their expressions in immune reactions by quantitative PCR analysis. These genes may potentially be used as molecular markers to distinguish between granulocytes and plasmatocytes in Ostrinia furnacalis, and further to clarify the functions of immune hemocytes in cellular immune reaction such as encapsulation and so on.
Identifiants
pubmed: 32700449
doi: 10.1111/1744-7917.12854
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1382-1398Subventions
Organisme : National Key R&D Program of China
ID : 2017YFD200400
Organisme : Guangdong Basic and Applied Basic Research Foundation
ID : 2020A1515010455
Informations de copyright
© 2020 Institute of Zoology, Chinese Academy of Sciences.
Références
Anderl, I., Vesala, L., Ihalainen, T.O., Vanha-Aho, L.M., Ando, I., Ramet, M. et al. (2016) Transdifferentiation and proliferation in two distinct hemocyte lineages in Drosophila melanogaster larvae after wasp infection. PLoS Pathogens, 12, e1005746.
Beetz, S., Brinkmann, M. and Trenczek, T. (2004) Differences between larval and pupal hemocytes of the tobacco hornworm, Manduea sexta, determined by monoclonal antibodies and density centrifugation. Journal of Insect Physiology, 50, 805-819.
Behrooz, A.B., Syahir, A. and Ahmad, S. (2019) CD133: beyond a cancer stem cell biomarker. Journal of Drug Targeting, 27, 257-269.
Belloncik, S., Petcharawan, O., Couillard, M., Charpentier, G., Larue, B., Guardado, H. et al. (2007) Development and characterization of a continuous cell line, AFKM-On-H, from hemocytes of the European corn borer Ostrinia nubilalis (Hübner) (Lepidoptera, Pyralidae). In Vitro Cellular & Developmental Biology-Animal, 43, 245-254.
Chisa, Y.A., Imanishi, S., Iiyama, K. and Kawarabata, T. (2004) Establishment of phagocytic cell lines from larval hemocytes of the beet armyworm, Spodoptera exigua. In Vitro Cellular & Developmental Biology-Animal, 40, 183-186.
Clark, K.D., Pech, L.L. and Strand, M.R. (1997) Isolation and identification of a plasmatocyte-spreading peptide from the hemolymph of the lepidopteran insect Pseudoplusia includens. Journal of Biological Chemistry, 272, 23440-23447.
Gardiner, E.M.M. and Strand, M.R. (1999) Monoclonal antibodies bind distinct classes of hemocytes in the moth Pseudoplusia includens. Journal of Insect Physiology, 45, 113-126.
Hilgen, G., Huebner, A.K., Tanimoto, N., Sothilingam, V., Seide, C., Garcia Garrido, M., et al. (2012) Lack of the sodium-driven chloride bicarbonate exchanger NCBE impairs visual function in the mouse retina. PLoS One, 7, e46155.
Hillyer, J.F. (2015) Insect immunology and hematopoiesis. Developmental and Comparative Immunology, 58, 102-118.
Hu, J., Feng, X.P., Yang, Z.G., Chen, Z.X. and Zhang, W.Q. (2014) A continuous cell line, SYSU-OfHe-C, from hemocytes of Ostrinia furnacalis possesses immune ability depending on the presence of larval plasma. Developmental and Comparative Immunology, 45, 10-20.
Johnson, J.A., Bitra, K., Zhang, S., Wang, L.H., Lynn, D.E. and Strand, M.R. (2010) The UGA-CiE1 cell line from Chrysodeixis includens exhibits characteristics of granulocytes and is permissive to infection by two viruses. Insect Biochemistry and Molecular Biology, 40, 394-404.
Kim, D., Pertea, G., Trapnell, C., Pimentel, H., Kelley, R. and Salzberg, S.L. (2013) TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions. Genome Biology, 14, R36.
Kim, Y., Jung, S. and Madanagopal, N. (2008) Antagonistic effect of juvenile hormone on hemocyte-spreading behavior of Spodoptera exigua in response to an insect cytokine and its putative membrane action. Journal of Insect Physiology, 54, 909-915.
Koiwai, K., Alenton, R.R.R., Shiomi, R., Nozaki, R., Kondo, H. and Hirono, I. (2017) Two hemocyte sub-populations of kuruma shrimp Marsupenaeus japonicus. Molecular Immunology, 85, 1-8.
Koiwai, K., Kondo, H. and Hirono, I. (2018) RNA-seq identifies integrin alpha of kuruma shrimp Marsupenaeus japonicus as a candidate molecular marker for phagocytic hemocytes. Developmental and Comparative Immunology, 81, 271-278.
Kurucz, E., Markus, R., Zsamboki, J., Folkl-Medzihradszky, K., Darula, Z., Vilmos, P. et al. (2007a) Nimrod, a putative phagocytosis receptor with EGF repeats in Drosophila plasmatocytes. Current Biology, 17, 649-654.
Kurucz, E., Vaczi, B., Markus, R., Laurinyecz, B., Vilmos, P., Zsamboki, J. et al. (2007b) Definition of Drosophila hemocyte subsets by cell-type specific antigens. Acta Biologica Hungarica, 58, 95-111.
Lapointe, J.F., Dunphy, G.B., Giannoulis, P., Mandato, C.A., Nardi, J.B., Gharib, O.H. et al. (2011) Cell lines, Md108 and Md66, from the hemocytes of Malacosoma disstria (Lepidoptera) display aspects of plasma-free innate non-self activities. Journal of Invertebrate Pathology, 108, 180-193.
Lehman, W. and Weilepp, M. (1989) Establishment of a cell line from hemocytes of Mamestra brassicae L. and its sensitivity to a homologous nuclear polyhedrosis virus (in German). Archiv Fuer Phytopathologie Und Pflanzenschultz, 25, 387-402.
Liu, W., Ma, P.W.K., Marsella-Herrick, P., Rosenfield, C.L., Knipple, D.C. and Roelofs, T. (1999) Cloning and functional expression of a cDNA encoding a metabolic acyl-CoA Delta 9-desaturase of the cabbage looper moth, Trichoplusia ni. Insect Biochemistry and Molecular Biology, 29, 435-443.
Livak, K.J. and Schmittgen, T.D. (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods, 25, 402-408.
Ma, Q., Cao, Z., Yu, Y.N., Yan, L.L., Zhang, W.J., Shi, Y. et al. (2017) Bombyx neuropeptide G protein-coupled receptor A7 is the third cognate receptor for short neuropeptide F from silkworm. Journal of Biological Chemistry, 292, 20599-20612.
Nakahara, Y., Kanamori, Y., Kiuchi, M. and Kamimura, M. (2010) Two hemocyte lineages exist in silkworm larval hematopoietic organ. PLoS ONE, 5, e11816.
Pech, L.L. and Strand, M.R. (1996) Granular cells are required for encapsulation of foreign targets by insect haemocytes. Journal of Cell Science, 109, 2053-2060.
Pech, L.L., Trudeau, D. and Strand, M.R. (1994) Separation and behavior in vitro of hemocytes from the moth, Pseudoplusia includens. Cell and Tissue Research, 277, 159-167.
Strand, M.R. and Pech, L.L. (1995) Immunological basis for compatibility in parasitoid-host relationships. Annual Review of Entomology, 40, 31-56.
Strand, M.R., Hayakawa, Y. and Clark, K.D. (2000) Plasmatocyte spreading peptide (PSP1) and growth blocking peptide (GBP) are multifunctional homologs. Journal of Insect Physiology, 46, 817-824.
Vilmos, P., Nagy, I., Kurucz, E., Hultmark, D., Gateff, E. and Ando, I. (2004) A rapid rosetting method for separation of hemocyte sub-populations of Drosophila melanogaster. Developmental and Comparative Immunology, 28, 555-563.
Wiegand, C., Levin, D., Gillespie, J.P., Willott, E., Kanost, M.R. and Trenczek, T. (2000) Monoclonal antibody MS13 identifies a plasmatocyte membrane protein and inhibits encapsulation and spreading reactions of Manduca sexta hemocytes. Archives of Insect Biochemistry and Physiology, 45, 95-108.
Willott, E., Trenczek, T., Thrower, L.W. and Kanost, M.R. (1994) Immunochemical identification of insect hemocyte populations: monoclonal antibodies distinguish four major hemocyte types in Manduca sexta. European Journal of Cell Biology, 65, 417-423.
Zhang, K., Tan, J., Xu, M., Su, J.J., Hu, R.J., Chen, Y.B. et al. (2014) A novel granulocyte-specific alpha integrin is essential for cellular immunity in the silkworm Bombyx mori. Journal of Insect Physiology, 71, 61-67.
Zhang, S., Clark, K.D. and Strand, M.R. (2011) The protein P23 identifies capsule-forming plasmatocytes in the moth Pseudoplusia includens. Developmental and Comparative Immunology, 35, 501-510.