Label-free discrimination and selection of cancer cells from blood during flow using holography-induced dielectrophoresis.

cell sorting circulating tumor cell dielectrophoresis imaging flow cytometry lab on a chip quantitative phase microscopy

Journal

Journal of biophotonics
ISSN: 1864-0648
Titre abrégé: J Biophotonics
Pays: Germany
ID NLM: 101318567

Informations de publication

Date de publication:
11 2020
Historique:
received: 21 04 2020
revised: 02 07 2020
accepted: 21 07 2020
pubmed: 24 7 2020
medline: 24 6 2021
entrez: 24 7 2020
Statut: ppublish

Résumé

We present a method for label-free imaging and sorting of cancer cells in blood, which is based on a dielectrophoretic microfluidic chip and label-free interferometric phase microscopy. The chip used for imaging has been embedded with dielectrophoretic electrodes, and therefore it can be used to sort the cells based on the decisions obtained during the cell flow by the label-free quantitative imaging method. Hence, we obtained a real-time, automatic, label-free imaging flow cytometry with the ability to sort the cells during flow. To validate our model, we combined into the label-free imaging interferometer a fluorescence imaging channel that indicated the correctness of the label-free sorting. We have achieved above 98% classification success and 69% sorting accuracy at flow rates of 4 to 7 μL hr

Identifiants

pubmed: 32700785
doi: 10.1002/jbio.202000151
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e202000151

Informations de copyright

© 2020 Wiley-VCH GmbH.

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Auteurs

Matan Dudaie (M)

Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv, Israel.

Noga Nissim (N)

Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv, Israel.

Itay Barnea (I)

Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv, Israel.

Tobias Gerling (T)

Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses, Potsdam, Germany.

Claus Duschl (C)

Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses, Potsdam, Germany.

Michael Kirschbaum (M)

Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses, Potsdam, Germany.

Natan T Shaked (NT)

Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv, Israel.

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