CRISPR/Cas9-mediated introduction of the sodium/iodide symporter gene enables noninvasive in vivo tracking of induced pluripotent stem cell-derived cardiomyocytes.
CRISPR/Cas9
NIS
PET
cardiomyocytes
electrophysiology
iPSCs
imaging
in vivo tracking
rhesus macaque
sodium iodide symporter
Journal
Stem cells translational medicine
ISSN: 2157-6580
Titre abrégé: Stem Cells Transl Med
Pays: England
ID NLM: 101578022
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
15
01
2020
revised:
10
05
2020
accepted:
24
05
2020
pubmed:
24
7
2020
medline:
2
9
2021
entrez:
24
7
2020
Statut:
ppublish
Résumé
Techniques that enable longitudinal tracking of cell fate after myocardial delivery are imperative for optimizing the efficacy of cell-based cardiac therapies. However, these approaches have been underutilized in preclinical models and clinical trials, and there is considerable demand for site-specific strategies achieving long-term expression of reporter genes compatible with safe noninvasive imaging. In this study, the rhesus sodium/iodide symporter (NIS) gene was incorporated into rhesus macaque induced pluripotent stem cells (RhiPSCs) via CRISPR/Cas9. Cardiomyocytes derived from NIS-RhiPSCs (NIS-RhiPSC-CMs) exhibited overall similar morphological and electrophysiological characteristics compared to parental control RhiPSC-CMs at baseline and with exposure to physiological levels of sodium iodide. Mice were injected intramyocardially with 2 million NIS-RhiPSC-CMs immediately following myocardial infarction, and serial positron emission tomography/computed tomography was performed with
Identifiants
pubmed: 32700830
doi: 10.1002/sctm.20-0019
pmc: PMC7519772
doi:
Substances chimiques
Symporters
0
sodium-iodide symporter
4XE5NDT4K1
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1203-1217Informations de copyright
© 2020 The Authors. STEM CELLS Translational Medicine published by Wiley Periodicals LLC on behalf of AlphaMed Press.
Références
Cell Stem Cell. 2008 Dec 4;3(6):587-90
pubmed: 19041774
Mol Ther Methods Clin Dev. 2014 Nov 12;1:14053
pubmed: 26052521
Curr Protoc Stem Cell Biol. 2017 May 16;41:4A.11.1-4A.11.13
pubmed: 28510330
Blood. 2004 Mar 1;103(5):1641-6
pubmed: 14604966
Mol Ther. 2012 May;20(5):1014-21
pubmed: 22434137
Nature. 2016 Oct 20;538(7625):388-391
pubmed: 27723741
J Am Coll Cardiol. 2008 Nov 11;52(20):1652-60
pubmed: 18992656
Nature. 2011 Mar 10;471(7337):225-9
pubmed: 21240260
EJNMMI Res. 2017 Dec;7(1):32
pubmed: 28378292
J Biol Chem. 1997 Oct 24;272(43):27230-8
pubmed: 9341168
Mol Ther. 2014 Apr;22(4):774-85
pubmed: 24441399
Annu Rev Physiol. 2017 Feb 10;79:261-289
pubmed: 28192058
J Nucl Med. 2017 Oct;58(10):1666-1671
pubmed: 28385795
Toxicol Sci. 2017 Jan;155(1):234-247
pubmed: 27701120
Circulation. 2011 Feb 1;123(4):425-43
pubmed: 21282520
EJNMMI Res. 2017 Oct 27;7(1):90
pubmed: 29080017
Stem Cells Transl Med. 2020 Oct;9(10):1203-1217
pubmed: 32700830
N Engl J Med. 2010 Oct 7;363(15):1397-409
pubmed: 20660394
J Nucl Med. 2006 Nov;47(11):1851-7
pubmed: 17079819
Biochim Biophys Acta. 1993 Jun 8;1154(1):65-82
pubmed: 8507647
Mol Ther. 2016 Apr;24(4):678-84
pubmed: 26867951
Nat Rev Cancer. 2011 Dec 01;12(1):51-8
pubmed: 22129804
Circulation. 2012 Jul 24;126(4):430-9
pubmed: 22767659
Nucl Med Biol. 2015 Jul;42(7):621-9
pubmed: 25899941
Nat Methods. 2014 Aug;11(8):855-60
pubmed: 24930130
Toxicol Sci. 2016 Dec;154(2):320-331
pubmed: 27621282
Stem Cells. 2013 May;31(5):829-37
pubmed: 23355363
J Am Coll Cardiol. 2014 Aug 5;64(5):436-48
pubmed: 25082575
Clin Nucl Med. 2018 Mar;43(3):162-167
pubmed: 29356744
J Am Coll Cardiol. 2016 May 10;67(18):2161-2176
pubmed: 27151349
Sci Rep. 2018 Apr 12;8(1):5907
pubmed: 29651156
Mol Ther. 2017 Jan 4;25(1):44-53
pubmed: 28129126
Circ Arrhythm Electrophysiol. 2017 Jun;10(6):
pubmed: 28630169
Stem Cell Reports. 2018 Feb 13;10(2):422-435
pubmed: 29398480
Cell Rep. 2014 May 22;7(4):1298-1309
pubmed: 24835994
Anal Bioanal Chem. 1996 Mar;354(5-6):576-9
pubmed: 15067448
Curr Protoc Stem Cell Biol. 2017 Nov 15;43:5A.11.1-5A.11.14
pubmed: 29140568
Nat Biotechnol. 2018 Aug;36(7):597-605
pubmed: 29969440
J Nucl Med. 2016 Sep;57(9):1454-9
pubmed: 27103021
Eur J Nucl Med Mol Imaging. 2010 Nov;37(11):2108-16
pubmed: 20577737
Circ Res. 2012 Oct 12;111(9):1125-36
pubmed: 22912385
Nature. 2014 Jun 12;510(7504):273-7
pubmed: 24776797