Molecular, physiological, and biochemical characterization of extracellular lipase production by
Aspergillus niger
Dendrogram
Lipase
Random Amplified Polymorphic DNA
Submerged fermentation method
Unweighted pair group method with arithmetical averages
Journal
PeerJ
ISSN: 2167-8359
Titre abrégé: PeerJ
Pays: United States
ID NLM: 101603425
Informations de publication
Date de publication:
2020
2020
Historique:
received:
22
12
2019
accepted:
05
06
2020
entrez:
25
7
2020
pubmed:
25
7
2020
medline:
25
7
2020
Statut:
epublish
Résumé
Extracellular production of fungal lipases especially the lipases obtained from the A total of 256 fungal isolates were obtained from oil seeds. From each genus, one isolate was selected to evaluate lipase production using phenol red and tributyrin plate assays. Lipase activity was estimated using the spectrophotometric pNPP hydrolysis assay. The highest lipase producer isolates were identified using 18S ribosomal RNA gene sequencing. The genetic variability was determined by random amplified polymorphic DNA (RAPD) analysis and the dendrogram was constructed using the unweighted pair group method with arithmetic averages method. The isolates were examined in a submerged fermentation culture (Smf) to measure the effect of temperature, pH, incubation time, carbon source, nitrogen source, inoculum volume, and lipid source on lipase production. Eleven isolates belonging to the genus The current study provides a comprehensive characterization of the optimal conditions, which are essential to enhance lipase production in five
Sections du résumé
BACKGROUND
BACKGROUND
Extracellular production of fungal lipases especially the lipases obtained from the
METHODS
METHODS
A total of 256 fungal isolates were obtained from oil seeds. From each genus, one isolate was selected to evaluate lipase production using phenol red and tributyrin plate assays. Lipase activity was estimated using the spectrophotometric pNPP hydrolysis assay. The highest lipase producer isolates were identified using 18S ribosomal RNA gene sequencing. The genetic variability was determined by random amplified polymorphic DNA (RAPD) analysis and the dendrogram was constructed using the unweighted pair group method with arithmetic averages method. The isolates were examined in a submerged fermentation culture (Smf) to measure the effect of temperature, pH, incubation time, carbon source, nitrogen source, inoculum volume, and lipid source on lipase production.
RESULTS
RESULTS
Eleven isolates belonging to the genus
CONCLUSIONS
CONCLUSIONS
The current study provides a comprehensive characterization of the optimal conditions, which are essential to enhance lipase production in five
Identifiants
pubmed: 32704444
doi: 10.7717/peerj.9425
pii: 9425
pmc: PMC7350912
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e9425Informations de copyright
©2020 Alabdalall et al.
Déclaration de conflit d'intérêts
The authors declare there are no competing interests.
Références
Indian J Microbiol. 2009 Mar;49(1):77-83
pubmed: 23100754
Environ Monit Assess. 2018 Sep 6;190(10):574
pubmed: 30191326
Int J Biol Macromol. 2018 Apr 1;109:389-398
pubmed: 29258898
Bioresour Technol. 2011 Apr;102(7):4909-12
pubmed: 21292479
Annu Rev Microbiol. 1999;53:315-51
pubmed: 10547694
Enzyme Microb Technol. 2000 Jun 1;26(9-10):657-663
pubmed: 10862870
Bioresour Technol. 2004 Jan;91(1):77-84
pubmed: 14585624
Bioresour Technol. 2016 Jul;211:224-30
pubmed: 27019125
Biotechnol Bioeng. 1991 Mar 5;37(5):467-83
pubmed: 18597393
Appl Microbiol Biotechnol. 2006 May;70(6):679-82
pubmed: 16170531
Appl Biochem Biotechnol. 2012 Jan;166(2):486-520
pubmed: 22072143
Bioresour Technol. 2001 Jan;76(1):23-7
pubmed: 11315806
Saudi J Biol Sci. 2018 Mar;25(3):409-417
pubmed: 29686504
J Food Sci Technol. 2016 Sep;53(9):3408-3423
pubmed: 27777447
Braz J Microbiol. 2015 Mar 04;45(4):1503-11
pubmed: 25763060
Protein Expr Purif. 2012 Mar;82(1):83-9
pubmed: 22155648
Lipids. 2015 Nov;50(11):1155-63
pubmed: 26216145
Biotechnol Adv. 2001 Dec;19(8):627-62
pubmed: 14550014
Lett Appl Microbiol. 2008 Jul;47(1):12-8
pubmed: 18498318
Enzyme Microb Technol. 2000 Jul 1;27(1-2):74-82
pubmed: 10862904