TUNEL assay-Standardized method for testing sperm DNA fragmentation.
DNA fragmentation
TUNEL assay
flow cytometer
male infertility
sperm
Journal
Andrologia
ISSN: 1439-0272
Titre abrégé: Andrologia
Pays: Germany
ID NLM: 0423506
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
10
04
2020
revised:
29
05
2020
accepted:
30
05
2020
pubmed:
25
7
2020
medline:
22
6
2021
entrez:
25
7
2020
Statut:
ppublish
Résumé
Sperm DNA integrity is important for normal functions such as fertilization, implantation, pregnancy and fetal development. Sperm DNA fragmentation (SDF) is more common in infertile men and may be responsible for poor reproductive function. Although there are a number of tests available to measure SDF, the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-nick end labelling TUNEL) assay using flow cytometry is becoming more popular to measure the sperm DNA fragmentation. It is a direct test that measures both single- and double- DNA strand breaks. In this review, we describe the protocol, quality control and measurement of sperm DNA fragmentation using a benchtop flow cytometer. We also briefly discuss the factors that can affect the results, challenges and clinical implications of TUNEL in assessing male infertility.
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13738Informations de copyright
© 2020 Blackwell Verlag GmbH.
Références
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