In-Vitro Identification and In-Vivo Confirmation of DNA Methylation Biomarkers for Urothelial Cancer.
DNA methylation
biomarkers
cell lines
primary cells
urine
urothelial cancer
Journal
Biomedicines
ISSN: 2227-9059
Titre abrégé: Biomedicines
Pays: Switzerland
ID NLM: 101691304
Informations de publication
Date de publication:
22 Jul 2020
22 Jul 2020
Historique:
received:
28
06
2020
revised:
19
07
2020
accepted:
20
07
2020
entrez:
26
7
2020
pubmed:
28
7
2020
medline:
28
7
2020
Statut:
epublish
Résumé
We identified DNA methylation targets specific for urothelial cancer (UC) by genome-wide methylation difference analysis of human urothelial (RT4, J82, 5637), prostate (LNCAP, DU-145, PC3) and renal (RCC-KP, CAKI-2, CAL-54) cancer cell lines with their respective primary epithelial cells. A large overlap of differentially methylated targets between all organs was observed and 40 Cytosine-phosphate-Guanine motifs (CpGs) were only specific for UC cells. Of those sites, two also showed high methylation differences (≥47%) in vivo when we further compared our data to those previously obtained in our array-based analyses of urine samples in 12 UC patients and 12 controls. Using mass spectrometry, we finally assessed seven CpG sites in this "bladder-specific" region of interest in urine samples of patients with urothelial (
Identifiants
pubmed: 32707764
pii: biomedicines8080233
doi: 10.3390/biomedicines8080233
pmc: PMC7459535
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : The federal ministry Federal Ministry of Innovation, Science and Research (North Rhine Westphalia, Germany)
ID : "Project PURE"
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