A Novel Multiplex qRT-PCR Assay to Detect SARS-CoV-2 Infection: High Sensitivity and Increased Testing Capacity.
COVID-19
SARS-CoV-2
direct multiplex qRT-PCR
multiplex qRT-PCR
qRT-PCR
Journal
Microorganisms
ISSN: 2076-2607
Titre abrégé: Microorganisms
Pays: Switzerland
ID NLM: 101625893
Informations de publication
Date de publication:
17 Jul 2020
17 Jul 2020
Historique:
received:
23
06
2020
revised:
13
07
2020
accepted:
14
07
2020
entrez:
26
7
2020
pubmed:
28
7
2020
medline:
28
7
2020
Statut:
epublish
Résumé
Rapid and sensitive screening of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential to limit the spread of the global pandemic we are facing. Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) is currently used for the clinical diagnosis of SARS-CoV-2 infection using nasopharyngeal swabs, tracheal aspirates, or bronchoalveolar lavage (BAL) samples. Despite the high sensitivity of the qRT-PCR method, false negative outcomes might occur, especially in patients with a low viral load. Here, we developed a multiplex qRT-PCR methodology for the simultaneous detection of SARS-CoV-2 genome (
Identifiants
pubmed: 32708870
pii: microorganisms8071064
doi: 10.3390/microorganisms8071064
pmc: PMC7409169
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Ricerca Sanitaria Finalizzata Giovani Ricercatori
ID : GR-2018-12365776
Organisme : Worldwide Cancer Research
ID : 20-0033
Pays : United Kingdom
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