Lipid composition modulates ATP hydrolysis and calcium phosphate mineral propagation by TNAP-harboring proteoliposomes.
Adenosine Triphosphate
/ metabolism
Alkaline Phosphatase
/ metabolism
Animals
Biomineralization
/ physiology
Calcium Phosphates
/ metabolism
Cattle
Cholesterol
/ chemistry
Dimyristoylphosphatidylcholine
/ chemistry
Hydrolysis
Liposomes
/ chemistry
Proteolipids
/ chemistry
Rats
Sphingomyelins
/ chemistry
Alkaline phosphatase
Biomineralization
Cholesterol
Matrix vesicle biomimetic systems
Proteoliposome
Sphingomyelin
Journal
Archives of biochemistry and biophysics
ISSN: 1096-0384
Titre abrégé: Arch Biochem Biophys
Pays: United States
ID NLM: 0372430
Informations de publication
Date de publication:
30 09 2020
30 09 2020
Historique:
received:
30
04
2020
revised:
09
06
2020
accepted:
22
06
2020
pubmed:
28
7
2020
medline:
25
11
2020
entrez:
26
7
2020
Statut:
ppublish
Résumé
Bone biomineralization is mediated by a special class of extracellular vesicles, named matrix vesicles (MVs), released by osteogenic cells. The MV membrane is enriched in sphingomyelin (SM), cholesterol (Chol) and tissue non-specific alkaline phosphatase (TNAP) compared with the parent cells' plasma membrane. TNAP is an ATP phosphohydrolase bound to cell and MV membranes via a glycosylphosphatidylinositol (GPI) anchor. Previous studies have shown that the lipid microenvironment influences the catalytic activity of enzymes incorporated into lipid bilayers. However, there is a lack of information about how the lipid microenvironment controls the ability of MV membrane-bound enzymes to induce mineral precipitation. Herein, we used TNAP-harboring proteoliposomes made of either pure dimyristoylphosphatidylcholine (DMPC) or DMPC mixed with either Chol, SM or both of them as MV biomimetic systems to evaluate how the composition modulates the lipid microenvironment and, in turn, TNAP incorporation into the lipid bilayer by means of calorimetry. These results were correlated with the proteoliposomes' catalytic activity and ability to induce the precipitation of amorphous calcium phosphate (ACP) in vitro. DMPC:SM proteoliposomes displayed the highest efficiency of mineral propagation, apparent affinity for ATP and substrate hydrolysis efficiency, which correlated with their highest degree of membrane organization (highest ΔH), among the tested proteoliposomes. Results obtained from turbidimetry and Fourier transformed infrared (FTIR) spectroscopy showed that the tested proteoliposomes induced ACP precipitation with the order DMPC:SM>DMPC:Chol:SM≈DMPC:Chol>DMPC which correlated with the lipid organization and the presence of SM in the proteoliposome membrane. Our study arises important insights regarding the physical properties and role of lipid organization in MV-mediated mineralization.
Identifiants
pubmed: 32710882
pii: S0003-9861(20)30491-4
doi: 10.1016/j.abb.2020.108482
pmc: PMC8390000
mid: NIHMS1732528
pii:
doi:
Substances chimiques
Calcium Phosphates
0
Liposomes
0
Proteolipids
0
Sphingomyelins
0
proteoliposomes
0
Adenosine Triphosphate
8L70Q75FXE
Cholesterol
97C5T2UQ7J
Alkaline Phosphatase
EC 3.1.3.1
Dimyristoylphosphatidylcholine
U86ZGC74V5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
108482Subventions
Organisme : NIDCR NIH HHS
ID : R01 DE012889
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
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