tBHP treatment as a model for cellular senescence and pollution-induced skin aging.
Antioxidants
/ metabolism
Cells, Cultured
Cellular Senescence
Environmental Pollution
/ adverse effects
Epidermis
/ pathology
Fibroblasts
/ drug effects
Glutathione
/ metabolism
Humans
Models, Theoretical
Oxidative Stress
/ physiology
Reactive Oxygen Species
/ metabolism
Skin
/ metabolism
Skin Aging
/ pathology
tert-Butylhydroperoxide
/ metabolism
cigarette smoke
collagen degradation
epidermal thinning
oxidative stress
Journal
Mechanisms of ageing and development
ISSN: 1872-6216
Titre abrégé: Mech Ageing Dev
Pays: Ireland
ID NLM: 0347227
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
31
03
2020
revised:
06
07
2020
accepted:
17
07
2020
pubmed:
28
7
2020
medline:
14
9
2021
entrez:
26
7
2020
Statut:
ppublish
Résumé
Accumulation of senescent cells promotes the development of age-related pathologies and deterioration. In human skin, senescent cells potentially impair structure and function by secreting a mixture of signaling molecules and proteases that influence neighboring cells and degrade extracellular matrix components, such as elastin and collagen. One of the key underlying mechanisms of senescence and extrinsic skin aging is the increase of intracellular reactive oxygen species and resulting oxidative stress. Tert-butyl hydroperoxide (tBHP) is a known inducer of oxidative stress and cellular damage, acting at least in part by depleting the antioxidant glutathione. Here, we provide a detailed characterization of tBHP-induced senescence in human dermal fibroblasts in monolayer culture. In addition, results obtained with more physiological experimental models revealed that tBHP treated 3D reconstructed skin and ex vivo skin developed signs of chronic tissue damage, displaying reduced epidermal thickness and collagen fiber thinning. We, therefore, propose that tBHP treatment can be used as a model to study the effects of extrinsic skin aging, focusing mainly on the influence of environmental pollution.
Identifiants
pubmed: 32710895
pii: S0047-6374(20)30114-7
doi: 10.1016/j.mad.2020.111318
pmc: PMC7610711
mid: EMS123020
pii:
doi:
Substances chimiques
Antioxidants
0
Reactive Oxygen Species
0
tert-Butylhydroperoxide
955VYL842B
Glutathione
GAN16C9B8O
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
111318Subventions
Organisme : Austrian Science Fund FWF
ID : J 4205
Pays : Austria
Organisme : Austrian Science Fund FWF
ID : P 31582
Pays : Austria
Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
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