Helicobacter pylori infection modulates endogenous hydrogen sulfide production in gastric cancer AGS cells.
Helicobacter pylori
cytotoxin-associated gene A
hydrogen sulfide
metabolomics
Journal
Helicobacter
ISSN: 1523-5378
Titre abrégé: Helicobacter
Pays: England
ID NLM: 9605411
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
13
03
2020
revised:
14
06
2020
accepted:
29
06
2020
pubmed:
28
7
2020
medline:
3
7
2021
entrez:
27
7
2020
Statut:
ppublish
Résumé
Persistent Helicobacter pylori infection induces gastric mucosal atrophy, which is a precancerous condition. Hydrogen sulfide (H Gastric cancer AGS cells were co-cultured with an H pylori standard strain possessing bacterial virulence factor CagA (ATCC 43504) and a strain without CagA (ATCC 51932). Three hours after inoculation, the cells were subjected to metabolomics analysis using gas chromatography-tandem mass spectrometry (GC-MS/MS). Orthogonal projections to latent structures discriminant analysis (OPLS-DA) and pathway analysis were performed. In addition, intracellular H Results of OPLS-DA showed a significant difference between the metabolism of untreated control cells and cells inoculated with the H pylori strains ATCC 51932 or ATCC 43504, mainly due to 45 metabolites. Pathway analysis with the selected metabolites indicated that methionine metabolism, which is related to H H pylori infection modulates endogenous H
Sections du résumé
BACKGROUND
BACKGROUND
Persistent Helicobacter pylori infection induces gastric mucosal atrophy, which is a precancerous condition. Hydrogen sulfide (H
MATERIALS AND METHODS
METHODS
Gastric cancer AGS cells were co-cultured with an H pylori standard strain possessing bacterial virulence factor CagA (ATCC 43504) and a strain without CagA (ATCC 51932). Three hours after inoculation, the cells were subjected to metabolomics analysis using gas chromatography-tandem mass spectrometry (GC-MS/MS). Orthogonal projections to latent structures discriminant analysis (OPLS-DA) and pathway analysis were performed. In addition, intracellular H
RESULTS
RESULTS
Results of OPLS-DA showed a significant difference between the metabolism of untreated control cells and cells inoculated with the H pylori strains ATCC 51932 or ATCC 43504, mainly due to 45 metabolites. Pathway analysis with the selected metabolites indicated that methionine metabolism, which is related to H
CONCLUSIONS
CONCLUSIONS
H pylori infection modulates endogenous H
Substances chimiques
Antigens, Bacterial
0
Bacterial Proteins
0
Hydrogen Sulfide
YY9FVM7NSN
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12732Informations de copyright
© 2020 John Wiley & Sons Ltd.
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