Photo-releasable derivatives of inositol pyrophosphates.
Cell entry
Inositol phosphate
Photo-activation
Prodrugs
Signaling
Journal
Methods in enzymology
ISSN: 1557-7988
Titre abrégé: Methods Enzymol
Pays: United States
ID NLM: 0212271
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
28
7
2020
pubmed:
28
7
2020
medline:
24
6
2021
Statut:
ppublish
Résumé
The specific non-invasive control of intracellular signaling events requires advanced tools that enter cells by diffusion and are controllable by light. Here, we detail the synthesis and application of membrane-permeant caged inositol pyrophosphates with respect to cell entry and cell distribution. We recently published the synthesis of these tools as well as their effect on PH-domain localization in HeLa cells and oscillations of the intracellular calcium concentration in β-cells, which are known to drive insulin secretion. In this chapter, we discuss the possibilities and limitations when using cell-penetrating inositol pyrophosphates. We provide a detailed protocol for the application in live mouse β-cells and we discuss the image analysis needed for following effects on calcium signaling.
Identifiants
pubmed: 32713537
pii: S0076-6879(20)30164-6
doi: 10.1016/bs.mie.2020.04.036
pii:
doi:
Substances chimiques
Diphosphates
0
Inositol Phosphates
0
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
53-73Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM127631
Pays : United States
Informations de copyright
© 2020 Elsevier Inc. All rights reserved.