Generation of a Retina Reporter hiPSC Line to Label Progenitor, Ganglion, and Photoreceptor Cell Types.
BRN3B
CRISPR
RCVRN
VSX2
hiPSCs
Journal
Translational vision science & technology
ISSN: 2164-2591
Titre abrégé: Transl Vis Sci Technol
Pays: United States
ID NLM: 101595919
Informations de publication
Date de publication:
18 02 2020
18 02 2020
Historique:
entrez:
28
7
2020
pubmed:
28
7
2020
medline:
28
7
2020
Statut:
epublish
Résumé
Early in mammalian eye development, CRISPR/Cas9-mediated homology-directed repair (HDR) in hiPSCs facilitated the replacement of the Retinal organoids formed from the PGP1 line expressed appropriate fluorescent proteins consistent with the differentiation of NRPs, RGCs, and PRs. Organoids produced from the PGP1 line expressed transcripts consistent with the development of all major retinal cell types. The PGP1 line offers a powerful new tool to study retinal development, retinal reprogramming, and therapeutic drug screening.
Identifiants
pubmed: 32714647
doi: 10.1167/tvst.9.3.21
pii: TVST-19-1751
pmc: PMC7352077
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Pagination
21Subventions
Organisme : NEI NIH HHS
ID : R01 EY026816
Pays : United States
Informations de copyright
Copyright 2020 The Authors.
Déclaration de conflit d'intérêts
Disclosure: P.T. Lam, PGP1 line (P); C. Gutierrez, None; K. Del Rio-Tsonis, PGP1 line (P); M.L. Robinson, PGP1 line (P)
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