SOD2 deficiency-induced oxidative stress attenuates steroidogenesis in mouse ovarian granulosa cells.
Animals
Antioxidants
/ metabolism
Catalase
/ metabolism
Cell Membrane
/ metabolism
Cells, Cultured
Cytosol
/ metabolism
Estradiol
/ biosynthesis
Female
Gene Expression Regulation
Glutathione Peroxidase
/ metabolism
Granulosa Cells
/ metabolism
Hydroxycholesterols
/ metabolism
Lipid Peroxidation
Mice, Inbred C57BL
Mitochondria
/ metabolism
Oxidative Stress
Progesterone
/ blood
Steroids
/ biosynthesis
Superoxide Dismutase
/ deficiency
Antioxidant enzymes
Estradiol
Lipid peroxidation
Progestin
Reactive oxygen species (ROS)
StAR protein
Journal
Molecular and cellular endocrinology
ISSN: 1872-8057
Titre abrégé: Mol Cell Endocrinol
Pays: Ireland
ID NLM: 7500844
Informations de publication
Date de publication:
01 01 2021
01 01 2021
Historique:
received:
07
11
2019
revised:
27
05
2020
accepted:
01
06
2020
pubmed:
28
7
2020
medline:
28
7
2021
entrez:
28
7
2020
Statut:
ppublish
Résumé
This study investigated the effects of SOD2 (MnSOD)-deficiency-induced excessive oxidative stress on ovarian steroidogenesis in vivo and isolated and cultured granulosa cells using WT and Sod2+/- mice. Basal and 48 h eCG-stimulated plasma progesterone levels were decreased ~50% in female Sod2+/- mice, whereas plasma progesterone levels were decreased ~70% in Sod2+/- mice after sequential stimulation with eCG followed by hCG. Sod2+/- deficiency caused about 50% reduction in SOD2 activity in granulosa cells. SOD2-deficiency also caused a marked reduction in progestins and estradiol in isolated granulosa cells. qRT-PCR measurements indicated that the mRNA expression levels of StAR protein and steroidogenic enzymes are decreased in the ovaries of Sod2+/- mice. Further studies showed a defect in the movement of mobilized cytosolic cholesterol to mitochondria. The ovarian membrane from Sod2+/- mice showed higher susceptibility to lipid peroxidation. These data indicates that SOD2-deficiency induced oxidative stress inhibits ovarian granulosa cell steroidogenesis primarily by interfering with cholesterol transport to mitochondria and attenuating the expression of Star protein gene and key steroidogenic enzyme genes.
Identifiants
pubmed: 32717420
pii: S0303-7207(20)30188-X
doi: 10.1016/j.mce.2020.110888
pmc: PMC8011630
mid: NIHMS1678509
pii:
doi:
Substances chimiques
Antioxidants
0
Hydroxycholesterols
0
Steroids
0
22-hydroxycholesterol
17711-16-9
Progesterone
4G7DS2Q64Y
Estradiol
4TI98Z838E
Catalase
EC 1.11.1.6
Glutathione Peroxidase
EC 1.11.1.9
Superoxide Dismutase
EC 1.15.1.1
superoxide dismutase 2
EC 1.15.1.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
110888Subventions
Organisme : BLRD VA
ID : I01 BX000398
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK116074
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Références
PLoS One. 2012;7(11):e49367
pubmed: 23145165
Nat Chem Biol. 2008 May;4(5):278-86
pubmed: 18421291
Curr Opin Obstet Gynecol. 2006 Jun;18(3):325-32
pubmed: 16735834
Methods Enzymol. 1984;105:121-6
pubmed: 6727660
J Steroid Biochem Mol Biol. 2004 Jan;88(1):61-7
pubmed: 15026084
Clin Interv Aging. 2018 Apr 26;13:757-772
pubmed: 29731617
J Reprod Fertil. 1995 Nov;105(2):253-7
pubmed: 8568768
Endocrinology. 1975 Nov;97(5):1328-31
pubmed: 1183412
Curr Opin Toxicol. 2018 Feb;7:9-16
pubmed: 29159324
Endocrinology. 1991 Jun;128(6):2958-66
pubmed: 2036971
Endocrinology. 1996 May;137(5):1580-4
pubmed: 8612488
Endocrinology. 1990 Jul;127(1):245-50
pubmed: 1694491
Annu Rev Biochem. 2017 Jun 20;86:749-775
pubmed: 28226215
Cell Signal. 2012 May;24(5):981-90
pubmed: 22286106
Anal Biochem. 1988 Oct;174(1):331-6
pubmed: 3064653
Endocrinology. 1991 May;128(5):2253-8
pubmed: 1708332
Methods Enzymol. 1978;52:302-10
pubmed: 672633
J Biol Chem. 1998 Oct 23;273(43):28510-5
pubmed: 9774481
Free Radic Biol Med. 2001 Nov 1;31(9):1101-10
pubmed: 11677043
Free Radic Biol Med. 2004 Mar 1;36(5):542-54
pubmed: 14980699
Free Radic Biol Med. 2005 Jun 1;38(11):1458-70
pubmed: 15890620
Antioxid Redox Signal. 2011 Sep 15;15(6):1583-606
pubmed: 21473702
Drug Metab Rev. 2006;38(1-2):171-96
pubmed: 16684656
Anal Biochem. 1978 Jun 15;87(1):206-10
pubmed: 98070
Eur J Med Chem. 2015 Jun 5;97:55-74
pubmed: 25942353
Nat Rev Drug Discov. 2004 Mar;3(3):205-14
pubmed: 15031734
J Gerontol A Biol Sci Med Sci. 2015 May;70(5):533-44
pubmed: 25016531
Antioxid Redox Signal. 2014 Apr 1;20(10):1599-617
pubmed: 23581847
Pediatr Res. 2009 Aug;66(2):121-7
pubmed: 19390491
Biochim Biophys Acta. 1988 Nov 25;963(2):139-50
pubmed: 2848582
Physiol Rev. 2014 Apr;94(2):329-54
pubmed: 24692350
Reprod Med Biol. 2005 Mar 07;4(1):31-44
pubmed: 29699208
Mol Cell. 2012 Jun 8;46(5):584-94
pubmed: 22681886
J Cell Biol. 2018 Jun 4;217(6):1915-1928
pubmed: 29669742
J Cell Biol. 2011 Jul 11;194(1):7-15
pubmed: 21746850
Endocrinology. 2003 Jul;144(7):2882-91
pubmed: 12810543
Nat Rev Endocrinol. 2013 Apr;9(4):228-40
pubmed: 23438835
J Clin Invest. 1995 Sep;96(3):1414-24
pubmed: 7657814
Reprod Sci. 2016 Dec;23(12):1656-1661
pubmed: 27821562
Curr Biol. 2014 May 19;24(10):R453-62
pubmed: 24845678
Nat Rev Urol. 2017 Aug;14(8):470-485
pubmed: 28508879
Biochim Biophys Acta. 2012 May;1822(5):794-814
pubmed: 22198225
Endocrinology. 1998 Sep;139(9):4008-11
pubmed: 9724058
Methods Enzymol. 1974;31:90-102
pubmed: 4370714
Endocrinology. 1993 Dec;133(6):2827-32
pubmed: 8243310
Annu Rev Biophys Biomol Struct. 2001;30:421-55
pubmed: 11441809
J Clin Endocrinol Metab. 1993 Feb;76(2):337-42
pubmed: 7679398
Endocrinology. 1994 Dec;135(6):2723-30
pubmed: 7988463
Free Radic Biol Med. 2016 Aug 4;99:120-127
pubmed: 27497909
Fertil Steril. 1999 Oct;72(4):720-6
pubmed: 10521117
Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1462-7
pubmed: 21220312
Endocrinology. 1994 Feb;134(2):588-95
pubmed: 7507829
Biochim Biophys Acta. 2007 Feb;1773(2):93-104
pubmed: 17023064
Obstet Med. 2016 Sep;9(3):113-6
pubmed: 27630746
Biol Reprod. 1998 Jul;59(1):208-15
pubmed: 9675014
Indian J Biochem Biophys. 2003 Oct;40(5):300-8
pubmed: 22900323
Biochem J. 2009 Jan 1;417(1):1-13
pubmed: 19061483
Vitam Horm. 2014;94:99-127
pubmed: 24388188
Cell Physiol Biochem. 2017;44(2):532-553
pubmed: 29145191
Biol Reprod. 2005 May;72(5):1134-41
pubmed: 15647453
Physiol Rev. 2002 Jan;82(1):47-95
pubmed: 11773609
Mol Hum Reprod. 2006 Nov;12(11):655-60
pubmed: 17005595
Toxicol Pathol. 2002 Nov-Dec;30(6):620-50
pubmed: 12512863
J Obstet Gynaecol (Tokyo 1995). 1995 Aug;21(4):401-9
pubmed: 8775911
Arch Biochem Biophys. 1999 Mar 1;363(1):91-7
pubmed: 10049502
Biochim Biophys Acta. 1990 Nov 12;1047(2):148-60
pubmed: 2174267
J Endocrinol. 2008 Jul;198(1):193-207
pubmed: 18417530
Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2278-83
pubmed: 11226230
J Physiol. 2003 Oct 15;552(Pt 2):335-44
pubmed: 14561818
Biochem Biophys Res Commun. 1976 Aug 23;71(4):952-8
pubmed: 971321
J Cell Biol. 1974 Nov;63(2 Pt 1):383-401
pubmed: 4371790
J Steroid Biochem Mol Biol. 2020 Mar;197:105521
pubmed: 31705961
J Reprod Fertil. 1993 Mar;97(2):347-51
pubmed: 8501704
Circ Res. 2018 Mar 16;122(6):877-902
pubmed: 29700084
Biochim Biophys Acta. 1999 Jan 4;1436(3):565-76
pubmed: 9989286
Front Pharmacol. 2014 Jul 01;5:151
pubmed: 25024695
Anal Biochem. 1989 May 15;179(1):8-18
pubmed: 2547324
Mol Cell Endocrinol. 2004 Dec 30;228(1-2):67-78
pubmed: 15541573
J Mol Endocrinol. 2014 Aug;53(1):1-16
pubmed: 24780837
J Lipid Res. 2016 Nov;57(11):1976-1986
pubmed: 27009116
J Nutr Biochem. 2004 Apr;15(4):210-9
pubmed: 15068814
World Allergy Organ J. 2012 Jan;5(1):9-19
pubmed: 23268465
Free Radic Biol Med. 2010 Mar 15;48(6):749-62
pubmed: 20045723