Characteristics of Circular RNA Expression Profiles of Porcine Granulosa Cells in Healthy and Atretic Antral Follicles.
antral follicular atresia
circRNA expression profiles
granulosa cell apoptosis
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
23 Jul 2020
23 Jul 2020
Historique:
received:
23
06
2020
revised:
13
07
2020
accepted:
18
07
2020
entrez:
29
7
2020
pubmed:
29
7
2020
medline:
17
2
2021
Statut:
epublish
Résumé
Circular RNAs (circRNAs) are thought to play essential roles in multiple biological processes, including apoptosis, an important process in antral follicle atresia. We aimed to investigate the potential involvement of circRNAs in granulosa cell apoptosis and thus antral follicle atresia. CircRNA expression profiles were generated from porcine granulosa cells isolated from healthy antral (HA) and atretic antral (AA) follicles. Over 9632 circRNAs were identified, of which 62 circRNAs were differentially expressed (DE-circRNAs). Back-splicing, RNase R resistance, and stability of DE-circRNAs were validated, and miRNA binding sites and related target genes were predicted. Two exonic circRNAs with low false discovery rate (FDR) high fold change, miRNA binding sites, and relevant biological functions-circ_CBFA2T2 and circ_KIF16B-were selected for further characterization. qRT-PCR and linear regression analysis confirmed expression and correlation of the targeted genes-the antioxidant gene
Identifiants
pubmed: 32717899
pii: ijms21155217
doi: 10.3390/ijms21155217
pmc: PMC7432752
pii:
doi:
Substances chimiques
RNA, Circular
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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