In-vitro Bactericidal Activity of a Novel Plant Source Plumeria pudica against Some Human and Fish Pathogenic Bacteria.
Animals
Anti-Bacterial Agents
/ isolation & purification
Apocynaceae
/ chemistry
Bacteria
/ drug effects
Bacterial Infections
/ drug therapy
Fish Diseases
/ drug therapy
Humans
Microbial Sensitivity Tests
Plant Extracts
/ isolation & purification
Plant Leaves
/ chemistry
Solvents
/ chemistry
Spectroscopy, Fourier Transform Infrared
FT-IR
GC-MS
In-vitro bactericidal activity
MBC
MIC
Plumeria pudica
Journal
Current drug discovery technologies
ISSN: 1875-6220
Titre abrégé: Curr Drug Discov Technol
Pays: United Arab Emirates
ID NLM: 101157212
Informations de publication
Date de publication:
2021
2021
Historique:
received:
04
04
2020
revised:
03
07
2020
accepted:
04
07
2020
pubmed:
29
7
2020
medline:
15
12
2021
entrez:
29
7
2020
Statut:
ppublish
Résumé
The only remedy for up surging problem of antibiotic resistance is the discovery of antibacterial agents of natural origin. The present study was aimed at finding antibacterial potential of crude and solvent extracts of mature leaves of Plumeria pudica. Antibacterial activity of three different solvent extracts were evaluated in four human and four fish pathogenic bacteria by measuring the zone of inhibition and determining Minimum Inhibitory Concentration and Minimum Bactericidal Concentration values. Standard antibiotics were used as positive control. Preliminary phytochemical screening of most effective extract i.e., ethyl acetate extract, Fourier Transform Infra Red analysis and GC-MS analysis of the Thin Layer Chromatographic (TLC) fraction of ethyl acetate extract were done meticulously. All experiments were done thrice and analyzed statistically. Crude leaf extracts and solvent extracts caused good inhibition of bacterial growth in all selected bacteria. Ethyl acetate extract showed highest inhibition zones in all tested strains with maximum inhibition (19.50±0.29 mm) in Escherichia coli (MTCC 739). MBC/MIC of the extracts indicated that all three solvent extracts were bactericidal. Preliminary phytochemical tests revealed the presence of tannins, steroids and alkaloids and FT-IR analysis revealed presence of many functional groups namely alcoholic, amide, amine salt and aldehyde groups. From the GC-MS analysis of TLC fraction of ethyl acetate extract, five different bioactive compounds e.g., 2,4-ditert -butylphenyl 5-hydroxypentanoate, Oxalic acid; allyl nonyl ester, 7,9-Ditert-butyl-1-oxaspiro(4,5)deca-6,9-diene- 2,8-dione, Dibutyl phthalate and 2,3,5,8-tetramethyl-decane were identified. Leaf extracts of P. pudica contain bioactive compounds that can be used as broad spectrum bactericidal agent.
Sections du résumé
BACKGROUND
The only remedy for up surging problem of antibiotic resistance is the discovery of antibacterial agents of natural origin.
OBJECTIVE
The present study was aimed at finding antibacterial potential of crude and solvent extracts of mature leaves of Plumeria pudica.
METHODS
Antibacterial activity of three different solvent extracts were evaluated in four human and four fish pathogenic bacteria by measuring the zone of inhibition and determining Minimum Inhibitory Concentration and Minimum Bactericidal Concentration values. Standard antibiotics were used as positive control. Preliminary phytochemical screening of most effective extract i.e., ethyl acetate extract, Fourier Transform Infra Red analysis and GC-MS analysis of the Thin Layer Chromatographic (TLC) fraction of ethyl acetate extract were done meticulously. All experiments were done thrice and analyzed statistically.
RESULTS
Crude leaf extracts and solvent extracts caused good inhibition of bacterial growth in all selected bacteria. Ethyl acetate extract showed highest inhibition zones in all tested strains with maximum inhibition (19.50±0.29 mm) in Escherichia coli (MTCC 739). MBC/MIC of the extracts indicated that all three solvent extracts were bactericidal. Preliminary phytochemical tests revealed the presence of tannins, steroids and alkaloids and FT-IR analysis revealed presence of many functional groups namely alcoholic, amide, amine salt and aldehyde groups. From the GC-MS analysis of TLC fraction of ethyl acetate extract, five different bioactive compounds e.g., 2,4-ditert -butylphenyl 5-hydroxypentanoate, Oxalic acid; allyl nonyl ester, 7,9-Ditert-butyl-1-oxaspiro(4,5)deca-6,9-diene- 2,8-dione, Dibutyl phthalate and 2,3,5,8-tetramethyl-decane were identified.
CONCLUSION
Leaf extracts of P. pudica contain bioactive compounds that can be used as broad spectrum bactericidal agent.
Identifiants
pubmed: 32718293
pii: CDDT-EPUB-108483
doi: 10.2174/1570163817666200727101300
doi:
Substances chimiques
Anti-Bacterial Agents
0
Plant Extracts
0
Solvents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
503-510Informations de copyright
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