Gd(DOTA)-grafted submicronic polysaccharide-based particles functionalized with fucoidan as potential MR contrast agent able to target human activated platelets.
Adult
Blood Platelets
/ chemistry
Cells, Cultured
Contrast Media
/ chemistry
Cross-Linking Reagents
/ chemistry
Dextrans
/ chemistry
Emulsions
/ chemistry
Gadolinium
/ chemistry
Healthy Volunteers
Heterocyclic Compounds
/ chemistry
Heterocyclic Compounds, 1-Ring
/ chemistry
Human Umbilical Vein Endothelial Cells
Humans
Macromolecular Substances
/ chemistry
Magnetic Resonance Imaging
/ methods
Nanoparticles
/ chemistry
Organometallic Compounds
/ chemistry
Particle Size
Platelet Activation
Polysaccharides
/ chemistry
Spectrometry, X-Ray Emission
/ methods
Thrombosis
/ diagnosis
Dextran
MRI
crosslinking
fucoidan
human platelets
submicronic particles
Journal
Carbohydrate polymers
ISSN: 1879-1344
Titre abrégé: Carbohydr Polym
Pays: England
ID NLM: 8307156
Informations de publication
Date de publication:
01 Oct 2020
01 Oct 2020
Historique:
received:
24
01
2020
revised:
12
05
2020
accepted:
13
05
2020
entrez:
29
7
2020
pubmed:
29
7
2020
medline:
15
4
2021
Statut:
ppublish
Résumé
Early detection of thrombotic events remains a big medical challenge. Dextran-based submicronic particles bearing Gd(DOTA) groups and functionalized with fucoidan have been produced via a simple and green water-in-oil emulsification/co-crosslinking process. Their capacity to bind to human activated platelets was evidenced in vitro as well as their cytocompatibility with human endothelial cells. The presence of Gd(DOTA) moieties was confirmed by elemental analysis and total reflection X-ray fluorescence (TRXF) spectrometry. Detailed characterization of particles was performed in terms of size distribution, morphology, and relaxation rates. In particular, longitudinal and transversal proton relaxivities were respectively 1.7 and 5.0 times higher than those of DOTAREM. This study highlights their potential as an MRI diagnostic platform for atherothrombosis.
Identifiants
pubmed: 32718599
pii: S0144-8617(20)30631-7
doi: 10.1016/j.carbpol.2020.116457
pii:
doi:
Substances chimiques
Contrast Media
0
Cross-Linking Reagents
0
Dextrans
0
Emulsions
0
Heterocyclic Compounds
0
Heterocyclic Compounds, 1-Ring
0
Macromolecular Substances
0
Organometallic Compounds
0
Polysaccharides
0
1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid
1HTE449DGZ
fucoidan
9072-19-9
gadolinium 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetate
99J2XUF1JT
Gadolinium
AU0V1LM3JT
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
116457Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.