Functional annotation of human long noncoding RNAs via molecular phenotyping.
Journal
Genome research
ISSN: 1549-5469
Titre abrégé: Genome Res
Pays: United States
ID NLM: 9518021
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
12
07
2019
accepted:
24
06
2020
pubmed:
29
7
2020
medline:
21
10
2021
entrez:
29
7
2020
Statut:
ppublish
Résumé
Long noncoding RNAs (lncRNAs) constitute the majority of transcripts in the mammalian genomes, and yet, their functions remain largely unknown. As part of the FANTOM6 project, we systematically knocked down the expression of 285 lncRNAs in human dermal fibroblasts and quantified cellular growth, morphological changes, and transcriptomic responses using Capped Analysis of Gene Expression (CAGE). Antisense oligonucleotides targeting the same lncRNAs exhibited global concordance, and the molecular phenotype, measured by CAGE, recapitulated the observed cellular phenotypes while providing additional insights on the affected genes and pathways. Here, we disseminate the largest-to-date lncRNA knockdown data set with molecular phenotyping (over 1000 CAGE deep-sequencing libraries) for further exploration and highlight functional roles for
Identifiants
pubmed: 32718982
pii: gr.254219.119
doi: 10.1101/gr.254219.119
pmc: PMC7397864
doi:
Substances chimiques
KCNQ Potassium Channels
0
KCNQ5 protein, human
0
Oligonucleotides, Antisense
0
RNA, Long Noncoding
0
RNA, Small Interfering
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1060-1072Subventions
Organisme : NCI NIH HHS
ID : P30 CA006973
Pays : United States
Organisme : Medical Research Council
ID : MC_UU_00007/16
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_00007/11
Pays : United Kingdom
Organisme : NCI NIH HHS
ID : R01 CA200859
Pays : United States
Organisme : Medical Research Council
ID : MC_UP_1102/1
Pays : United Kingdom
Informations de copyright
© 2020 Ramilowski et al.; Published by Cold Spring Harbor Laboratory Press.
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