Olfactory ensheathing cells seeded decellularized scaffold promotes axonal regeneration in spinal cord injury rats.
Animals
Axons
/ physiology
Biomarkers
Cells, Cultured
Coculture Techniques
Gait Disorders, Neurologic
/ etiology
Gliosis
/ etiology
Materials Testing
Neuroglia
/ physiology
Olfactory Bulb
/ cytology
Random Allocation
Rats
Rats, Sprague-Dawley
Spinal Cord
Spinal Cord Injuries
/ complications
Spinal Cord Regeneration
Tissue Scaffolds
axonal regeneration
decellularized spinal scaffolds
olfactory ensheathing cells
scaffold
spinal cord injury
Journal
Journal of biomedical materials research. Part A
ISSN: 1552-4965
Titre abrégé: J Biomed Mater Res A
Pays: United States
ID NLM: 101234237
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
received:
01
05
2020
revised:
09
07
2020
accepted:
11
07
2020
pubmed:
29
7
2020
medline:
19
1
2022
entrez:
29
7
2020
Statut:
ppublish
Résumé
Spinal cord decellularized (DC) scaffolds can promote axonal regeneration and restore hindlimb motor function of spinal cord defect rats. However, scarring caused by damage to the astrocytes at the margin of injury can hinder axon regeneration. Olfactory ensheathing cells (OECs) integrate and migrate with astrocytes at the site of spinal cord injury, providing a bridge for axons to penetrate the scars and grow into lesion cores. The purpose of this study was to evaluate whether DC scaffolds carrying OECs could better promote axon growth. For these studies, DC scaffolds were cocultured with primary extracted and purified OECs. Immunofluorescence and electron microscopy were used for verification of cells adhere and growth on the scaffold. Scaffolds with OECs were transplanted into rat spinal cord defects to evaluate axon regeneration and functional recovery of hind limbs. Basso, Beattie, and Bresnahan (BBB) scoring was used to assess motor function recovery, and glial fibrillary acidic protein (GFAP) and NF200-stained tissue sections were used to evaluate axonal regeneration and astrological scar distribution. Our results indicated that spinal cord DC scaffolds have good histocompatibility and spatial structure, and can promote the proliferation of adherent OECs. In animal experiments, scaffolds carrying OECs have better axon regeneration promoting protein expression than the SCI model, and improve the proliferation and distribution of astrocytes at the site of injury. These results proved that the spinal cord DC scaffold with OECs can promote axon regeneration at the site of injury, providing a new basis for clinical application.
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
779-787Informations de copyright
© 2020 Wiley Periodicals LLC.
Références
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