Stability of d-luciferin for bioluminescence to detect gene expression in freely moving mice for long durations.
Period1
circadian rhythm
in vivo imaging
luciferin
Journal
Luminescence : the journal of biological and chemical luminescence
ISSN: 1522-7243
Titre abrégé: Luminescence
Pays: England
ID NLM: 100889025
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
12
06
2020
revised:
14
07
2020
accepted:
20
07
2020
pubmed:
29
7
2020
medline:
29
7
2021
entrez:
29
7
2020
Statut:
ppublish
Résumé
Circadian disturbance of clock gene expression is a risk factor for diseases such as obesity, cancer, and sleep disorders. To study these diseases, it is necessary to monitor and analyze the expression rhythm of clock genes in the whole body for a long duration. The bioluminescent reporter enzyme firefly luciferase and its substrate d-luciferin have been used to generate optical signals from tissues in vivo with high sensitivity. However, little information is known about the stability of d-luciferin to detect gene expression in living animals for a long duration. In the present study, we examined the stability of a luciferin solution over 21 days. l-Luciferin, which is synthesized using racemization of d-luciferin, was at high concentrations after 21 days. In addition, we showed that bioluminescence of Period1 (Per1) expression in the liver was significantly decreased compared with the day 1 solution, although locomotor activity rhythm was not affected. These results showed that d-luciferin should be applied to the mouse within, at most, 7 days to detect bioluminescence of Per1 gene expression rhythm in vivo.
Substances chimiques
Benzothiazoles
0
D-luciferin
0
Firefly Luciferin
5TBB02N29K
Luciferases, Firefly
EC 1.13.12.7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
94-98Subventions
Organisme : JSPS KAKENHI
ID : 17H04022
Organisme : JSPS KAKENHI
ID : 18H04724
Organisme : 'Resonance Bio'
Informations de copyright
© 2020 John Wiley & Sons, Ltd.
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