Recent Advances of In Vitro Culture for the Application of New Breeding Techniques in Citrus.

agroinfiltration disease resistance genome editing genotype promoter regeneration selectable-marker genes transformation

Journal

Plants (Basel, Switzerland)
ISSN: 2223-7747
Titre abrégé: Plants (Basel)
Pays: Switzerland
ID NLM: 101596181

Informations de publication

Date de publication:
24 Jul 2020
Historique:
received: 06 07 2020
revised: 21 07 2020
accepted: 22 07 2020
entrez: 30 7 2020
pubmed: 30 7 2020
medline: 30 7 2020
Statut: epublish

Résumé

Citrus is one of the most important fruit crops in the world. This review will discuss the recent findings related to citrus transformation and regeneration protocols of juvenile and adult explants. Despite the many advances that have been made in the last years (including the use of inducible promoters and site-specific recombination systems), transformation efficiency, and regeneration potential still represent a bottleneck in the application of the new breeding techniques in commercial citrus varieties. The influence of genotype, explant type, and other factors affecting the regeneration and transformation of the most used citrus varieties will be described, as well as some examples of how these processes can be applied to improve fruit quality and resistance to various pathogens and pests, including the potential of using genome editing in citrus. The availability of efficient regeneration and transformation protocols, together with the availability of the source of resistance, is made even more important in light of the fast diffusion of emerging diseases, such as Huanglongbing (HLB), which is seriously challenging citriculture worldwide.

Identifiants

pubmed: 32722179
pii: plants9080938
doi: 10.3390/plants9080938
pmc: PMC7465985
pii:
doi:

Types de publication

Journal Article Review

Langues

eng

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Auteurs

Lara Poles (L)

Food and Environment (Di3A), Department of Agriculture, University of Catania, Via Valdisavoia 5, 95123 Catania, Italy.
CREA, Research Centre for Olive, Fruit and Citrus Crops, Corso Savoia 190, 95024 Acireale, Italy.

Concetta Licciardello (C)

CREA, Research Centre for Olive, Fruit and Citrus Crops, Corso Savoia 190, 95024 Acireale, Italy.

Gaetano Distefano (G)

Food and Environment (Di3A), Department of Agriculture, University of Catania, Via Valdisavoia 5, 95123 Catania, Italy.

Elisabetta Nicolosi (E)

Food and Environment (Di3A), Department of Agriculture, University of Catania, Via Valdisavoia 5, 95123 Catania, Italy.

Alessandra Gentile (A)

Food and Environment (Di3A), Department of Agriculture, University of Catania, Via Valdisavoia 5, 95123 Catania, Italy.
College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, China.

Stefano La Malfa (S)

Food and Environment (Di3A), Department of Agriculture, University of Catania, Via Valdisavoia 5, 95123 Catania, Italy.

Classifications MeSH