Effective pressure and treatment duration of high hydrostatic pressure to prepare melanoma vaccines.

HHP effective pressure immunogenicity melanoma vaccine treatment duration

Journal

Oncology letters
ISSN: 1792-1074
Titre abrégé: Oncol Lett
Pays: Greece
ID NLM: 101531236

Informations de publication

Date de publication:
Aug 2020
Historique:
received: 15 10 2019
accepted: 06 04 2020
entrez: 30 7 2020
pubmed: 30 7 2020
medline: 30 7 2020
Statut: ppublish

Résumé

Current therapeutic methods for melanoma have numerous limitations, and thus the improvement of such treatment methods are essential. One possible option is the vaccination of autologous inactivated tumor cells. The primary indispensable principles of a cell-based melanoma vaccine include: i) Entire inactivation of melanoma cells; ii) retaining the immunogenicity of melanoma cells; and iii) adherence to laws and ethical guidelines. However, traditional methods for the production of the vaccine, such as ultrasonic, chemotherapeutics and freeze-thawing, have some juridical or therapeutic constraints. Therefore, the present study used high hydrostatic pressure (HHP) to inactivate malignant cells, and treated B16-F10 tumor cells with different pressures (≥50 MPa) and different durations (≥1 min). It was identified that tumor cells

Identifiants

pubmed: 32724353
doi: 10.3892/ol.2020.11657
pii: OL-0-0-11657
pmc: PMC7377178
doi:

Types de publication

Journal Article

Langues

eng

Pagination

1135-1142

Informations de copyright

Copyright: © Liu et al.

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Auteurs

Kai Liu (K)

Department of Hand and Foot Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.

Shuai Yan (S)

Department of Operating Room, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.

Zhanchuan Ma (Z)

Institute of Immunology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.

Bin Liu (B)

Department of Hand and Foot Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.

Classifications MeSH