Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis.
Animals
Antigens, Bacterial
/ immunology
Apoptosis
Cattle
Cattle Diseases
/ immunology
Cell Proliferation
Coinfection
Cytokines
/ metabolism
Fasciola hepatica
/ immunology
Immunity
Leukocytes, Mononuclear
/ immunology
Macrophages
/ immunology
Mycobacterium avium subsp. paratuberculosis
/ immunology
Paratuberculosis
/ immunology
Th1 Cells
/ immunology
Johne’s disease
co-infection
fasciolosis
ileocaecal lymphocytes
immunoregulation
macrophages
peripheral blood mononuclear cell
Journal
Parasite immunology
ISSN: 1365-3024
Titre abrégé: Parasite Immunol
Pays: England
ID NLM: 7910948
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
23
04
2020
revised:
17
06
2020
accepted:
17
07
2020
pubmed:
30
7
2020
medline:
5
3
2021
entrez:
30
7
2020
Statut:
ppublish
Résumé
Fasciola hepatica causes economically important disease in livestock worldwide. The relevance of this parasitic infection extends beyond its direct consequences due to its immunoregulatory properties. Given the importance of the T helper 1 (Th1) immune response in controlling infections with Mycobacterium avium subspecies paratuberculosis (MAP) in cattle, we aimed to establish the immunological consequences that co-infection with F. hepatica might have on the course of Johne's disease (JD). This study compared the in vitro response of bovine immune cells to infection with MAP or exposure to MAP antigens following F. hepatica infection or stimulation with F. hepatica products. We found a decreased proliferation of peripheral blood mononuclear cells (PBMCs) after infection with F. hepatica. This reduction was inversely correlated with fluke burden. Pre-stimulation with F. hepatica molecules produced a significant reduction of ileocaecal lymph node leucocyte proliferation in response to MAP antigens. Additionally,F. hepatica products reduced expression of the CD14 receptor by macrophages and increased levels of apoptosis and bacterial (MAP) uptake. Overall, F. hepatica infection had little impact on the in vitro response of immune cells to MAP, whereas in vitro co-stimulation with F. hepatica molecules had a measurable effect. Whether this is likely to affect JD progression during in vivo chronic conditions remains unclear.
Sections du résumé
BACKGROUND
Fasciola hepatica causes economically important disease in livestock worldwide. The relevance of this parasitic infection extends beyond its direct consequences due to its immunoregulatory properties.
OBJECTIVES
Given the importance of the T helper 1 (Th1) immune response in controlling infections with Mycobacterium avium subspecies paratuberculosis (MAP) in cattle, we aimed to establish the immunological consequences that co-infection with F. hepatica might have on the course of Johne's disease (JD).
METHODS
This study compared the in vitro response of bovine immune cells to infection with MAP or exposure to MAP antigens following F. hepatica infection or stimulation with F. hepatica products.
RESULTS
We found a decreased proliferation of peripheral blood mononuclear cells (PBMCs) after infection with F. hepatica. This reduction was inversely correlated with fluke burden. Pre-stimulation with F. hepatica molecules produced a significant reduction of ileocaecal lymph node leucocyte proliferation in response to MAP antigens. Additionally,F. hepatica products reduced expression of the CD14 receptor by macrophages and increased levels of apoptosis and bacterial (MAP) uptake.
CONCLUSIONS
Overall, F. hepatica infection had little impact on the in vitro response of immune cells to MAP, whereas in vitro co-stimulation with F. hepatica molecules had a measurable effect. Whether this is likely to affect JD progression during in vivo chronic conditions remains unclear.
Identifiants
pubmed: 32725900
doi: 10.1111/pim.12779
pmc: PMC8365740
doi:
Substances chimiques
Antigens, Bacterial
0
Cytokines
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12779Subventions
Organisme : Science Foundation Ireland
ID : 14/IA/2304
Pays : Ireland
Informations de copyright
© 2020 The Authors. Parasite Immunology published by John Wiley & Sons Ltd.
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