Active Lysogeny in Listeria Monocytogenes Is a Bacteria-Phage Adaptive Response in the Mammalian Environment.
active lysogeny
bacterial pathogen
listeria
mammalian infection
phage
phage adaptaion
phage excision
phage transcriptional response
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
28 07 2020
28 07 2020
Historique:
received:
07
02
2020
revised:
05
05
2020
accepted:
02
07
2020
entrez:
30
7
2020
pubmed:
30
7
2020
medline:
11
5
2021
Statut:
ppublish
Résumé
Some Listeria monocytogenes (Lm) strains harbor a prophage within the comK gene, which renders it inactive. During Lm infection of macrophage cells, the prophage turns into a molecular switch, promoting comK gene expression and therefore Lm intracellular growth. During this process, the prophage does not produce infective phages or cause bacterial lysis, suggesting it has acquired an adaptive behavior suited to the pathogenic lifestyle of its host. In this study, we demonstrate that this non-classical phage behavior, named active lysogeny, relies on a transcriptional response that is specific to the intracellular niche. While the prophage undergoes lytic induction, the process is arrested midway, preventing the transcription of the late genes. Further, we demonstrate key phage factors, such as LlgA transcription regulator and a DNA replicase, that support the phage adaptive behavior. This study provides molecular insights into the adaptation of phages to their pathogenic hosts, uncovering unusual cooperative interactions.
Identifiants
pubmed: 32726621
pii: S2211-1247(20)30937-2
doi: 10.1016/j.celrep.2020.107956
pmc: PMC7397523
pii:
doi:
Substances chimiques
Bacterial Proteins
0
ComK protein, Listeria monocytogenes
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
107956Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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