Human ex vivo spinal cord slice culture as a useful model of neural development, lesion, and allogeneic neural cell therapy.
Human organotypic culture
Spinal cord injury
Stem cell therapy
Journal
Stem cell research & therapy
ISSN: 1757-6512
Titre abrégé: Stem Cell Res Ther
Pays: England
ID NLM: 101527581
Informations de publication
Date de publication:
29 07 2020
29 07 2020
Historique:
received:
27
02
2020
accepted:
12
06
2020
revised:
18
05
2020
entrez:
31
7
2020
pubmed:
31
7
2020
medline:
22
6
2021
Statut:
epublish
Résumé
There are multiple promising treatment strategies for central nervous system trauma and disease. However, to develop clinically potent and safe treatments, models of human-specific conditions are needed to complement in vitro and in vivo animal model-based studies. We established human brain stem and spinal cord (cross- and longitudinal sections) organotypic cultures (hOCs) from first trimester tissues after informed consent by donor and ethical approval by the Regional Human Ethics Committee, Stockholm (lately referred to as Swedish Ethical Review Authority), and The National Board of Health and Welfare, Sweden. We evaluated the stability of hOCs with a semi-quantitative hOC score, immunohistochemistry, flow cytometry, Ca The spinal cord hOCs presented relatively stable features during 7-21 days in vitro (DIV) (except a slightly increased cell proliferation and activated glial response). After contusion injury performed at 7 DIV, a significant reduction of the hOC score, increase of the activated caspase-3 We conclude that human spinal cord slice cultures have potential for future structural and functional studies of human spinal cord development, injury, and treatment strategies.
Sections du résumé
BACKGROUND
There are multiple promising treatment strategies for central nervous system trauma and disease. However, to develop clinically potent and safe treatments, models of human-specific conditions are needed to complement in vitro and in vivo animal model-based studies.
METHODS
We established human brain stem and spinal cord (cross- and longitudinal sections) organotypic cultures (hOCs) from first trimester tissues after informed consent by donor and ethical approval by the Regional Human Ethics Committee, Stockholm (lately referred to as Swedish Ethical Review Authority), and The National Board of Health and Welfare, Sweden. We evaluated the stability of hOCs with a semi-quantitative hOC score, immunohistochemistry, flow cytometry, Ca
RESULTS
The spinal cord hOCs presented relatively stable features during 7-21 days in vitro (DIV) (except a slightly increased cell proliferation and activated glial response). After contusion injury performed at 7 DIV, a significant reduction of the hOC score, increase of the activated caspase-3
CONCLUSIONS
We conclude that human spinal cord slice cultures have potential for future structural and functional studies of human spinal cord development, injury, and treatment strategies.
Identifiants
pubmed: 32727554
doi: 10.1186/s13287-020-01771-y
pii: 10.1186/s13287-020-01771-y
pmc: PMC7390865
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
320Commentaires et corrections
Type : ErratumIn
Références
Exp Neurol. 2001 Aug;170(2):305-16
pubmed: 11476597
Neurosurg Rev. 2012 Jul;35(3):293-311; discussion 311
pubmed: 22539011
Curr Opin Neurol. 2012 Jun;25(3):322-33
pubmed: 22547103
Glia. 2010 Jul;58(9):1118-32
pubmed: 20468053
Brain Res Brain Res Protoc. 2002 Oct;10(2):60-8
pubmed: 12431704
Nat Med. 2018 May;24(4):484-490
pubmed: 29480894
Methods Mol Biol. 2019;1936:169-183
pubmed: 30820899
Exp Neurol. 2014 Mar;253:138-45
pubmed: 24412492
Stem Cell Res. 2013 May;10(3):325-37
pubmed: 23416350
J Neurosci Res. 2006 Aug 15;84(3):471-82
pubmed: 16721767
Microsc Res Tech. 1999 Jun 15;45(6):359-82
pubmed: 10402264
Glia. 2012 Mar;60(3):441-56
pubmed: 22139636
Exp Neurol. 1998 Jan;149(1):262-76
pubmed: 9454636
Expert Opin Biol Ther. 2017 May;17(5):529-541
pubmed: 28306359
Neuropharmacology. 2013 Aug;71:46-55
pubmed: 23542439
Nat Med. 2007 May;13(5):561-6
pubmed: 17479102
Exp Cell Res. 2017 Aug 15;357(2):236-242
pubmed: 28587745
Neuroscience. 2016 May 13;322:377-97
pubmed: 26917272
J Neuroimmunol. 2005 Apr;161(1-2):1-11
pubmed: 15748938
J Neurosci Res. 2001 Jun 1;64(5):533-41
pubmed: 11391708
Immunity. 2016 Mar 15;44(3):505-515
pubmed: 26982357
Neural Regen Res. 2019 Jan;14(1):7-19
pubmed: 30531063
J Neurosci Methods. 1981 Dec;4(4):329-42
pubmed: 7033675
Stem Cells Transl Med. 2016 Jul;5(7):914-24
pubmed: 27130222
J Neurosci. 2002 Aug 1;22(15):6623-30
pubmed: 12151541
J Biomater Appl. 2014 Feb;28(6):825-36
pubmed: 23674184
World Neurosurg. 2011 Mar-Apr;75(3-4):533-9
pubmed: 21600508
Expert Opin Ther Targets. 2011 Apr;15(4):505-18
pubmed: 21281256
Stem Cell Res. 2009 Jan;2(1):56-67
pubmed: 19383409
Kennedy Inst Ethics J. 2012 Sep;22(3):243-61
pubmed: 23285793
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Biology (Basel). 2020 Mar 15;9(3):
pubmed: 32183469
J Neurosci Methods. 1991 Apr;37(2):173-82
pubmed: 1715499
Stem Cells. 2010 Jan;28(1):93-9
pubmed: 19904738
Elife. 2018 Oct 15;7:
pubmed: 30320555
J Comp Neurol. 2006 Jul 10;497(2):199-208
pubmed: 16705680
C R Seances Soc Biol Fil. 1954 Aug-Sep;148(15-18):1493-5
pubmed: 14352543
Expert Opin Drug Discov. 2012 Jun;7(6):525-34
pubmed: 22607235
Eur J Neurosci. 2008 May;27(10):2483-92
pubmed: 18513321
Cold Spring Harb Perspect Biol. 2015 Jul 01;7(8):a020537
pubmed: 26134003
J Neurosci. 2009 Mar 11;29(10):3276-87
pubmed: 19279265
Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2160-5
pubmed: 16461457
Tissue Cell. 2019 Feb;56:90-97
pubmed: 30736910
Brain Res. 2007 Mar 16;1137(1):50-7
pubmed: 17223088
Ann Neurol. 2012 Oct;72(4):491-501
pubmed: 23109144
Prog Neurobiol. 2009 Aug;88(4):221-45
pubmed: 19552996
Exp Neurobiol. 2010 Sep;19(2):106-13
pubmed: 22110349
Drug Discov Today. 2010 Jun;15(11-12):436-43
pubmed: 20346411
Front Cell Neurosci. 2011 Jun 17;5:9
pubmed: 21734866
Biomaterials. 2014 May;35(14):4288-96
pubmed: 24565523
Neuroscience. 2015 Oct 1;305:86-98
pubmed: 26254240
Brain Res Bull. 1995;38(4):383-91
pubmed: 8535861
J Neurosci. 2004 Feb 18;24(7):1726-33
pubmed: 14973255
Trends Neurosci. 2006 Oct;29(10):547-53
pubmed: 16938356
Oncology. 1975;31(3-4):244-53
pubmed: 1196583
Biomaterials. 2014 Mar;35(9):2617-29
pubmed: 24406216
Folia Biol (Praha). 2016;62(6):263-267
pubmed: 28189150
J Exp Biol. 2010 May;213(Pt 9):1513-20
pubmed: 20400636
Exp Neurol. 2020 May;327:113232
pubmed: 32044329
Spine (Phila Pa 1976). 2012 Jan 15;37(2):E73-8
pubmed: 21685825
Dev Neurobiol. 2015 Apr;75(4):360-8
pubmed: 25652687
J Neurosci Methods. 2002 Jun 30;117(2):159-66
pubmed: 12100981
Physiol Behav. 2007 Sep 10;92(1-2):60-6
pubmed: 17610915
Front Immunol. 2018 Mar 02;9:405
pubmed: 29552013
Cell Stem Cell. 2018 Jun 01;22(6):941-950.e6
pubmed: 29859175
Adv Exp Med Biol. 2012;760:53-73
pubmed: 23281513