Baicalin attenuated Mycoplasma gallisepticum-induced immune impairment in chicken bursa of fabricius through modulation of autophagy and inhibited inflammation and apoptosis.
Animals
Apoptosis
/ drug effects
Autophagy
/ drug effects
Bursa of Fabricius
/ cytology
Chickens
Flavonoids
/ administration & dosage
Mitochondria
/ genetics
Mycoplasma Infections
/ drug therapy
Mycoplasma gallisepticum
/ physiology
NF-kappa B
/ genetics
Oxidative Stress
/ drug effects
Poultry Diseases
/ drug therapy
Mycoplasma gallisepticum
apoptosis
baicalin
bursa of fabricius
immune impairment
Journal
Journal of the science of food and agriculture
ISSN: 1097-0010
Titre abrégé: J Sci Food Agric
Pays: England
ID NLM: 0376334
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
21
01
2020
revised:
05
07
2020
accepted:
29
07
2020
pubmed:
31
7
2020
medline:
30
3
2021
entrez:
31
7
2020
Statut:
ppublish
Résumé
Mycoplasma gallisepticum (MG) is the primary etiologic agent of chronic respiratory disease in poultry. However, the mechanism underlying MG-induced immune dysregulation in chicken is still elusive. Baicalin shows excellent anti-bacterial, anti-inflammatory, anti-carcinogenic and anti-viral properties. In the present study, the preventive effects of baicalin against immune impairment in chicken bursa of fabricius (BF) were studied in an MG infection model. Histopathological examination showed increased inflammatory cell infiltrations and fragmented nuclei in the model group. Ultrastructural analysis revealed the phenomenon of apoptosis in bursal cells, along with the deformation of mitochondrial membrane and swollen mitochondria in the model group. However, these abnormal morphological changes were partially alleviated by baicalin. Meanwhile, baicalin treatment attenuated the level of proinflammatory cytokines, and suppressed nuclear factor-kappa B expression at both protein and mRNA level. Terminal deoxynucleotidyl transferase-mediated dUTP nick endlabeling assay showed extensive apoptosis in BF in the model group. The mRNA and protein expression levels of apoptosis-related genes were upregulated in BF, while baicalin treatment significantly alleviated apoptosis in BF. In addition, alterations in mRNA and protein expression levels of autophagy-related genes and mitochondrial dynamics proteins were significantly alleviated by baicalin. Moreover, baicalin treatment significantly attenuated MG-induced decrease in CD8 These results suggested that baicalin could effectively inhibit MG-induced immune impairment and alleviate inflammatory responses and apoptosis in chicken BF. © 2020 Society of Chemical Industry.
Sections du résumé
BACKGROUND
BACKGROUND
Mycoplasma gallisepticum (MG) is the primary etiologic agent of chronic respiratory disease in poultry. However, the mechanism underlying MG-induced immune dysregulation in chicken is still elusive. Baicalin shows excellent anti-bacterial, anti-inflammatory, anti-carcinogenic and anti-viral properties. In the present study, the preventive effects of baicalin against immune impairment in chicken bursa of fabricius (BF) were studied in an MG infection model.
RESULTS
RESULTS
Histopathological examination showed increased inflammatory cell infiltrations and fragmented nuclei in the model group. Ultrastructural analysis revealed the phenomenon of apoptosis in bursal cells, along with the deformation of mitochondrial membrane and swollen mitochondria in the model group. However, these abnormal morphological changes were partially alleviated by baicalin. Meanwhile, baicalin treatment attenuated the level of proinflammatory cytokines, and suppressed nuclear factor-kappa B expression at both protein and mRNA level. Terminal deoxynucleotidyl transferase-mediated dUTP nick endlabeling assay showed extensive apoptosis in BF in the model group. The mRNA and protein expression levels of apoptosis-related genes were upregulated in BF, while baicalin treatment significantly alleviated apoptosis in BF. In addition, alterations in mRNA and protein expression levels of autophagy-related genes and mitochondrial dynamics proteins were significantly alleviated by baicalin. Moreover, baicalin treatment significantly attenuated MG-induced decrease in CD8
CONCLUSIONS
CONCLUSIONS
These results suggested that baicalin could effectively inhibit MG-induced immune impairment and alleviate inflammatory responses and apoptosis in chicken BF. © 2020 Society of Chemical Industry.
Substances chimiques
Flavonoids
0
NF-kappa B
0
baicalin
347Q89U4M5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
880-890Subventions
Organisme : The National Natural Science Foundation of China
ID : 31772801
Organisme : The National Natural Science Foundation of China
ID : 31802241
Organisme : Project of Youth Innovative Talent Training Program in Heilongjiang Province
ID : UNPYSCT-2018146
Organisme : Academic Backbone Project of Northeast Agricultural University
ID : 18XG23
Informations de copyright
© 2020 Society of Chemical Industry.
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