PVDF Nanofiber Scaffold Coated with a Vitronectin Peptide Facilitates the Neural Differentiation of Human Embryonic Stem Cells.
Dual-SMAD inhibition
Nanofiber
Neural differentiation
Polyvinylidene fluoride (PVDF)
Vitronectin
hESCs
Journal
Development & reproduction
ISSN: 2465-9525
Titre abrégé: Dev Reprod
Pays: Korea (South)
ID NLM: 101178352
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
20
04
2020
revised:
08
05
2020
accepted:
16
05
2020
entrez:
1
8
2020
pubmed:
1
8
2020
medline:
1
8
2020
Statut:
ppublish
Résumé
Polyvinylidene fluoride (PVDF) is a stable and biocompatible material that has been broadly used in biomedical applications. Due to its piezoelectric property, the electrospun nanofiber of PVDF has been used to culture electroactive cells, such as osteocytes and cardiomyocytes. Here, taking advantage of the piezoelectric property of PVDF, we have fabricated a PVDF nanofiber scaffolds using an electrospinning technique for differentiating human embryonic stem cells (hESCs) into neural precursors (NPs). Surface coating with a peptide derived from vitronectin enables hESCs to firmly adhere onto the nanofiber scaffolds and differentiate into NPs under dual-SMAD inhibition. Our nanofiber scaffolds supported the differentiation of hESCs into SOX1-positive NPs more significantly than Matrigel. The NPs generated on the nanofiber scaffolds could give rise to neurons, astrocytes, and oligodendrocyte precursors. Furthermore, comparative transcriptome analysis revealed the variable expressions of 27 genes in the nanofiber scaffold groups, several of which are highly related to the biological processes required for neural differentiation. These results suggest that a PVDF nanofiber scaffold coated with a vitronectin peptide can serve as a highly efficient and defined culture platform for the neural differentiation of hESCs.
Identifiants
pubmed: 32734130
doi: 10.12717/DR.2020.24.2.135
pii: dr-24-2-135
pmc: PMC7375977
doi:
Types de publication
Journal Article
Langues
eng
Pagination
135-147Commentaires et corrections
Type : ErratumIn
Informations de copyright
© Copyright 2020 The Korean Society of Developmental Biology.
Déclaration de conflit d'intérêts
The authors declare no potential conflict of interest.
Références
World J Stem Cells. 2014 Jan 26;6(1):11-23
pubmed: 24567784
Genome Biol. 2014;15(12):550
pubmed: 25516281
Nat Commun. 2015 Mar 25;6:6626
pubmed: 25806427
J Cell Biol. 2009 Feb 23;184(4):597-609
pubmed: 19221194
Physiology (Bethesda). 2018 Jan 1;33(1):16-25
pubmed: 29212889
Acta Biomater. 2018 Jul 15;75:129-139
pubmed: 29879551
Biomed Mater. 2015 Jan 29;10(1):015018
pubmed: 25634427
Curr Protoc Protein Sci. 2004 Nov;Chapter 6:Unit 6.3
pubmed: 18429271
Nat Commun. 2019 Apr 3;10(1):1523
pubmed: 30944313
Nat Biotechnol. 2009 Mar;27(3):275-80
pubmed: 19252484
Mater Sci Eng C Mater Biol Appl. 2017 Sep 1;78:1195-1202
pubmed: 28575957
Stem Cells Int. 2015;2015:167025
pubmed: 26351461
Nat Biotechnol. 2010 Jun;28(6):606-10
pubmed: 20512120
Cell Stem Cell. 2018 Mar 1;22(3):302-305
pubmed: 29499149
Stem Cell Rev Rep. 2010 Jun;6(2):270-81
pubmed: 20376579
Biomaterials. 2009 Feb;30(4):556-64
pubmed: 18977025
Nat Methods. 2010 Dec;7(12):989-94
pubmed: 21076418
Nat Neurosci. 2001 Nov;4 Suppl:1161-8
pubmed: 11687825
Annu Rev Mater Res. 2011 Aug 1;41:99-132
pubmed: 22058660
Nanomaterials (Basel). 2019 Jun 30;9(7):
pubmed: 31261995
Genes Dev. 2005 May 1;19(9):1022-7
pubmed: 15879552
Nat Biotechnol. 2001 Dec;19(12):1129-33
pubmed: 11731781
Nature. 2011 Nov 06;480(7378):547-51
pubmed: 22056989
F1000Res. 2015 Dec 30;4:1521
pubmed: 26925227
Proc Natl Acad Sci U S A. 2004 Aug 24;101(34):12543-8
pubmed: 15310843
J Cell Sci. 2013 Jul 15;126(Pt 14):3082-94
pubmed: 23641068
Biomaterials. 2007 Oct;28(28):4039-46
pubmed: 17574667
Nat Methods. 2017 Apr;14(4):417-419
pubmed: 28263959