Two Japanese pepper (Zanthoxylum piperitum) fruit-derived compounds attenuate IgE-mediated allergic response in vitro and in vivo via inhibition of mast cell degranulation.
Animals
Anti-Allergic Agents
/ pharmacology
Calcimycin
/ pharmacology
Cell Degranulation
/ drug effects
Cell Line
/ drug effects
Cell Survival
/ drug effects
Fruit
/ chemistry
Hypersensitivity
/ drug therapy
Immunoglobulin E
/ drug effects
Male
Mast Cells
/ drug effects
Mice
Mice, Inbred BALB C
Mice, Inbred ICR
Passive Cutaneous Anaphylaxis
/ drug effects
Rats
Zanthoxylum
/ chemistry
Anti-allergic activity
Degranulation
Mast cell
RBL-2H3
Zanthoxylum piperitum
Journal
European journal of pharmacology
ISSN: 1879-0712
Titre abrégé: Eur J Pharmacol
Pays: Netherlands
ID NLM: 1254354
Informations de publication
Date de publication:
15 Oct 2020
15 Oct 2020
Historique:
received:
01
08
2019
revised:
27
07
2020
accepted:
27
07
2020
pubmed:
4
8
2020
medline:
19
5
2021
entrez:
4
8
2020
Statut:
ppublish
Résumé
Zanthoxylum piperitum (ZP, 'Japanese pepper') is a traditional medicine and pepper used in Asian countries such as Japan. Hydroxy-α-sanshool, a pungent-tasting substance contained within ZP, has been reported to slightly suppress immunoglobulin E (IgE)-mediated mast cell degranulation. The current study aims to newly identify anti-allergic compounds derived from ZP. We examine the inhibitory mechanisms behind IgE-mediated mast cell degranulation. By inhibitory effect-guided isolation, we identified degranulation inhibitory compounds derived from ZP fruit: 1-acetoxy-7-hydroxy-3, 7-dimethylocta-2E, 5E-diene (ZP1) and 8-hydroxygeranyl acetate (ZP2). ZP1 and ZP2 inhibited IgE-mediated degranulation and A23187-mediated degranulation in RBL-2H3 mast cells. Our findings suggest the inhibition of degranulation by ZP1 and ZP2 was by inhibition of Lyn phosphorylation, followed by inhibition of intracellular Ca
Identifiants
pubmed: 32745602
pii: S0014-2999(20)30527-6
doi: 10.1016/j.ejphar.2020.173435
pii:
doi:
Substances chimiques
Anti-Allergic Agents
0
Immunoglobulin E
37341-29-0
Calcimycin
37H9VM9WZL
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
173435Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.