Motility based assays using cultured fourth stage larvae fail to provide consistent discrimination between known avermectin-resistant and -susceptible isolates of Cooperia spp.
Anthelmintic resistance
Avermectin
Cooperia
Diagnostic parasitology
Larval motility assay
Journal
Veterinary parasitology
ISSN: 1873-2550
Titre abrégé: Vet Parasitol
Pays: Netherlands
ID NLM: 7602745
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
08
10
2019
revised:
25
07
2020
accepted:
27
07
2020
pubmed:
4
8
2020
medline:
29
12
2020
entrez:
4
8
2020
Statut:
ppublish
Résumé
The fecal egg count reduction test (FECRT) is the only method commonly used for diagnosing anthelmintic resistance in gastrointestinal nematodes of cattle, but this method has several drawbacks that have limited its widescale implementation. Consequently, there exists a need to develop better methods for diagnosing resistance. Assays based on larval motility are used commonly for screening potential drug candidates, and for detecting drug resistance, but previous work in our lab demonstrated that the L3 stage failed to discriminate between avermectin-resistant and susceptible isolates of Cooperia spp. We hypothesized that the L4 may be a better stage for this purpose because it is a parasitic and actively feeding life stage without a double cuticle. L3 larvae of Cooperia spp. were exsheathed and cultured to L4 by maintaining them in media at 37 °C and 20 % CO
Identifiants
pubmed: 32745924
pii: S0304-4017(20)30177-1
doi: 10.1016/j.vetpar.2020.109197
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
109197Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.