De novo engineering of intracellular condensates using artificial disordered proteins.
Journal
Nature chemistry
ISSN: 1755-4349
Titre abrégé: Nat Chem
Pays: England
ID NLM: 101499734
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
12
11
2019
accepted:
18
06
2020
pubmed:
5
8
2020
medline:
9
2
2021
entrez:
5
8
2020
Statut:
ppublish
Résumé
Phase separation of intrinsically disordered proteins (IDPs) is a remarkable feature of living cells to dynamically control intracellular partitioning. Despite the numerous new IDPs that have been identified, progress towards rational engineering in cells has been limited. To address this limitation, we systematically scanned the sequence space of native IDPs and designed artificial IDPs (A-IDPs) with different molecular weights and aromatic content, which exhibit variable condensate saturation concentrations and temperature cloud points in vitro and in cells. We created A-IDP puncta using these simple principles, which are capable of sequestering an enzyme and whose catalytic efficiency can be manipulated by the molecular weight of the A-IDP. These results provide a robust engineered platform for creating puncta with new, phase-separation-mediated control of biological function in living cells.
Identifiants
pubmed: 32747754
doi: 10.1038/s41557-020-0511-7
pii: 10.1038/s41557-020-0511-7
pmc: PMC8281385
mid: NIHMS1719785
doi:
Substances chimiques
Intrinsically Disordered Proteins
0
Recombinant Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
814-825Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM127042
Pays : United States
Commentaires et corrections
Type : CommentIn
Type : CommentIn
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