The Antibody Society's antibody validation webinar series.
Antibody specificity
antibody selectivity
antibody validation
monoclonal antibodies
peptide antibodies
polyclonal antibodies
recombinant antibodies
reproducibility
selective antibodies
specific antibodies
Journal
mAbs
ISSN: 1942-0870
Titre abrégé: MAbs
Pays: United States
ID NLM: 101479829
Informations de publication
Date de publication:
Historique:
entrez:
5
8
2020
pubmed:
5
8
2020
medline:
6
7
2021
Statut:
ppublish
Résumé
In the wake of the reproducibility crisis and numerous discussions on how commercially available antibodies as research tool contribute to it, The Antibody Society developed a series of 10 webinars to address the issues involved. The webinars were delivered by speakers with both academic and commercial backgrounds. This report highlights the problems, and offers solutions to help the scientific community appropriately identify the right antibodies and to validate them for their research and development projects. Despite the various solutions proposed here, they must be applied on a case-by-case basis. Each antibody must be verified based on the content of the product sheet, and subsequently through experimentation to confirm integrity, specificity and selectivity. Verification needs to focus on the precise application and tissue/cell type for which the antibody will be used, and all verification data must be reported openly. The various approaches discussed here all have caveats, so a combination of solutions must be considered.
Identifiants
pubmed: 32748696
doi: 10.1080/19420862.2020.1794421
pmc: PMC7531563
doi:
Substances chimiques
Antibodies, Monoclonal
0
Types de publication
Journal Article
Video-Audio Media
Webcast
Langues
eng
Sous-ensembles de citation
IM
Pagination
1794421Subventions
Organisme : NIGMS NIH HHS
ID : R41 GM131551
Pays : United States
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