A Pilot Study of Chronological Microbiota Changes in a Rat Apical Periodontitis Model.
In vivo micro-computed tomography
Microbiota
Rat apical periodontitis model
apical periodontitis
Journal
Microorganisms
ISSN: 2076-2607
Titre abrégé: Microorganisms
Pays: Switzerland
ID NLM: 101625893
Informations de publication
Date de publication:
02 Aug 2020
02 Aug 2020
Historique:
received:
29
06
2020
revised:
27
07
2020
accepted:
30
07
2020
entrez:
5
8
2020
pubmed:
5
8
2020
medline:
5
8
2020
Statut:
epublish
Résumé
Apical periodontitis caused by microbial infection in the dental pulp is characterized by inflammation, destruction of the pulpal and periradicular tissues, and alveolar bone resorption. We analyzed the chronological changes in microbiota using a pyrosequencing-based approach combined with radiologic and histopathologic changes in a rat apical periodontitis model. During the three-week observation, the pulp and periapical area showed a typical progress of apical periodontitis. A total of 27 phyla, 645 genera, and 1276 species were identified. The root apex had a lower bacterial species diversity than the pulp chamber. Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria were dominant phyla in both the pulp chamber and root apex. Remarkably, bacterial communities showed a tendency to change in the root apex based on the disease progression. At the genus level,
Identifiants
pubmed: 32748824
pii: microorganisms8081174
doi: 10.3390/microorganisms8081174
pmc: PMC7464309
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : National Research Foundation of Korea
ID : NRF-2017M3A9E4047246, NRF-2018R1A5A2024418, NRF-2019R1A2C2007041, and NRF-2019R1I1A1A01060952
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