Comprehensive analyses of B-cell compartments across the human body reveal novel subsets and a gut-resident memory phenotype.
Journal
Blood
ISSN: 1528-0020
Titre abrégé: Blood
Pays: United States
ID NLM: 7603509
Informations de publication
Date de publication:
10 12 2020
10 12 2020
Historique:
received:
30
08
2019
accepted:
22
06
2020
pubmed:
5
8
2020
medline:
7
4
2021
entrez:
5
8
2020
Statut:
ppublish
Résumé
Although human B cells have been extensively studied, most reports have used peripheral blood as a source. Here, we used a unique tissue resource derived from healthy organ donors to deeply characterize human B-cell compartments across multiple tissues and donors. These datasets revealed that B cells in the blood are not in homeostasis with compartments in other tissues. We found striking donor-to-donor variability in the frequencies and isotype of CD27+ memory B cells (MBCs). A comprehensive antibody-based screen revealed markers of MBC and allowed identification of novel MBC subsets with distinct functions defined according to surface expression of CD69 and CD45RB. We defined a tissue-resident MBC phenotype that was predominant in the gut but absent in blood. RNA-sequencing of MBC subsets from multiple tissues revealed a tissue-resident MBC gene signature as well as gut- and spleen-specific signatures. Overall, these studies provide novel insights into the nature and function of human B-cell compartments across multiple tissues.
Identifiants
pubmed: 32750113
pii: S0006-4971(20)72811-9
doi: 10.1182/blood.2019002782
pmc: PMC7731793
doi:
Substances chimiques
Antigens, CD
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2774-2785Subventions
Organisme : NIAID NIH HHS
ID : P01 AI106697
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI145064
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI142744
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2020 by The American Society of Hematology.
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