Application of recombinase polymerase amplification method for rapid detection of infectious laryngotracheitis virus.
Detection
Infectious laryngotracheitis virus
Real-time
Recombinase polymerase amplification
Journal
Molecular and cellular probes
ISSN: 1096-1194
Titre abrégé: Mol Cell Probes
Pays: England
ID NLM: 8709751
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
21
05
2020
revised:
24
07
2020
accepted:
01
08
2020
pubmed:
8
8
2020
medline:
26
8
2021
entrez:
8
8
2020
Statut:
ppublish
Résumé
Infectious laryngotracheitis is a significant respiratory disease of chickens that causes huge economic losses due to high morbidity and mortality and reduced egg production. A real-time recombinase polymerase amplification (RPA) assay was developed to accurately detect ILTV. The specific probe and primer sets were carefully designed and screened. The real-time RPA assay was carried out at 39 °C for 30 min, and results were obtained within 15 min. The results of the specificity assay showed no fluorescence signals with other avian-related viruses. The sensitivity of the assay was 1 × 10
Identifiants
pubmed: 32758643
pii: S0890-8508(20)30327-3
doi: 10.1016/j.mcp.2020.101646
pii:
doi:
Substances chimiques
DNA Primers
0
Recombinases
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101646Informations de copyright
Copyright © 2020. Published by Elsevier Ltd.