CytoPAN-Portable cellular analyses for rapid point-of-care cancer diagnosis.

Biopsy, Fine-Needle Breast Neoplasms / diagnosis Female Humans Point-of-Care Systems Prospective Studies Sensitivity and Specificity

Journal

Science translational medicine
ISSN: 1946-6242
Titre abrégé: Sci Transl Med
Pays: United States
ID NLM: 101505086

Informations de publication

Date de publication:
05 08 2020
Historique:
received: 24 10 2019
revised: 06 03 2020
accepted: 19 06 2020
entrez: 8 8 2020
pubmed: 8 8 2020
medline: 24 6 2021
Statut: ppublish

Résumé

Rapid, automated, point-of-care cellular diagnosis of cancer remains difficult in remote settings due to lack of specialists and medical infrastructure. To address the need for same-day diagnosis, we developed an automated image cytometry system (CytoPAN) that allows rapid breast cancer diagnosis of scant cellular specimens obtained by fine needle aspiration (FNA) of palpable mass lesions. The system is devoid of moving parts for stable operations, harnesses optimized antibody kits for multiplexed analysis, and offers a user-friendly interface with automated analysis for rapid diagnoses. Through extensive optimization and validation using cell lines and mouse models, we established breast cancer diagnosis and receptor subtyping in 1 hour using as few as 50 harvested cells. In a prospective patient cohort study (

Identifiants

DOI: 10.1126/scitranslmed.aaz9746 PMID: 32759277 PMC: PMC8217912
pubmed: 32759277
pii: 12/555/eaaz9746
doi: 10.1126/scitranslmed.aaz9746
pmc: PMC8217912
mid: NIHMS1710856
pii:
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NCI NIH HHS
ID : R33 CA202064
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA229777
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA206997
Pays : United States
Organisme : NCI NIH HHS
ID : R00 CA201248
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA204019
Pays : United States
Organisme : NCI NIH HHS
ID : R21 CA217662
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA233360
Pays : United States
Organisme : NCI NIH HHS
ID : UH3 CA202637
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA206890
Pays : United States

Commentaires et corrections

Type : CommentIn

Informations de copyright

Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

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Auteurs

Jouha Min (J)

Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114, USA.
ORCID: 0000-0002-6737-7254

Lip Ket Chin (LK)

Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114, USA.
School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 639798, Singapore.
ORCID: 0000-0001-9020-7782

Juhyun Oh (J)

Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114, USA.
ORCID: 0000-0002-4492-6978

Christian Landeros (C)

Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114, USA.
Harvard-MIT Program in Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
ORCID: 0000-0002-6529-253X

Claudio Vinegoni (C)

Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114, USA.
ORCID: 0000-0002-9818-3639

Jeeyeon Lee (J)

Department of Surgery, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok Hospital, Daegu 41404, Republic of Korea.
ORCID: 0000-0003-1826-1690

Soo Jung Lee (SJ)

Department of Oncology/Hematology, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok Hospital, Daegu 41404, Republic of Korea.
ORCID: 0000-0003-0066-4109

Jee Young Park (JY)

Department of Pathology, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok Hospital, Daegu 41404, Republic of Korea.
ORCID: 0000-0002-1857-813X

Ai-Qun Liu (AQ)

School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 639798, Singapore.
ORCID: 0000-0002-0126-5778

Cesar M Castro (CM)

Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114, USA.
Cancer Center, Massachusetts General Hospital, Boston, MA 02114, USA.
ORCID: 0000-0002-1159-5658

Hakho Lee (H)

Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114, USA. rweissleder@mgh.harvard.edu im.hyungsoon@mgh.harvard.edu hlee@mgh.harvard.edu.
Department of Radiology, Massachusetts General Hospital, Boston, MA 02114, USA.
ORCID: 0000-0002-0087-0909

Hyungsoon Im (H)

Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114, USA. rweissleder@mgh.harvard.edu im.hyungsoon@mgh.harvard.edu hlee@mgh.harvard.edu.
Department of Radiology, Massachusetts General Hospital, Boston, MA 02114, USA.
ORCID: 0000-0002-0626-1346

Ralph Weissleder (R)

Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114, USA. rweissleder@mgh.harvard.edu im.hyungsoon@mgh.harvard.edu hlee@mgh.harvard.edu.
Department of Radiology, Massachusetts General Hospital, Boston, MA 02114, USA.
Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
ORCID: 0000-0003-0828-4143

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Cytodiagnostic › Biopsie › Ponction-biopsie à l'aiguille › Cytoponction › CytoPAN-Portable cellular analyses for rapid...
questionsmedicales.fr › Maladies de la peau et du tissu conjonctif › Maladies de la peau › Maladies du sein › Tumeurs du sein › CytoPAN-Portable cellular analyses for rapid...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › CytoPAN-Portable cellular analyses for rapid...
questionsmedicales.fr › Administration des services de santé › Gestion des soins aux patients › Systèmes automatisés lit malade › CytoPAN-Portable cellular analyses for rapid...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Caractéristiques des études épidémiologiques › Études épidémiologiques › Études de cohortes › Études prospectives › CytoPAN-Portable cellular analyses for rapid...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › CytoPAN-Portable cellular analyses for rapid...