Determinants of the Nucleotide Specificity in the Carbohydrate Epimerase Family 1.
CDP-paratose 2-epimerase
UDP-glucose 4-epimerase
carbohydrate epimerase
nucleotide sugar
rational mutagenesis
Journal
Biotechnology journal
ISSN: 1860-7314
Titre abrégé: Biotechnol J
Pays: Germany
ID NLM: 101265833
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
29
05
2020
revised:
20
07
2020
pubmed:
8
8
2020
medline:
16
3
2021
entrez:
8
8
2020
Statut:
ppublish
Résumé
In recent years, carbohydrate epimerases have attracted increasing attention as promising biocatalysts for the production of specialty sugars and derivatives. The vast majority of these enzymes are active on nucleotide-activated sugars, rather than on their free counterparts. Although such epimerases are known to have a clear preference for a particular nucleotide (UDP, GDP, CDP, or ADP), very little is known about the determinants of the respective specificities. In this work, sequence motifs are identified that correlate with the different nucleotide specificities in one of the main epimerase superfamilies, carbohydrate epimerase 1 (CEP1). To confirm their relevance, GDP- and CDP-specific residues are introduced into the UDP-glucose 4-epimerase from Thermus thermophilus, resulting in a 3-fold and 13-fold reduction in K
Identifiants
pubmed: 32761842
doi: 10.1002/biot.202000132
doi:
Substances chimiques
Nucleotides
0
Racemases and Epimerases
EC 5.1.-
Carbohydrate Epimerases
EC 5.1.3.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2000132Subventions
Organisme : S.V.O.
ID : FWO.3F0.2016.0056.01
Organisme : EpiSwitch project
ID : G0F3417N
Informations de copyright
© 2020 Wiley-VCH GmbH.
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