Distinct Phases of Postnatal Skeletal Muscle Growth Govern the Progressive Establishment of Muscle Stem Cell Quiescence.
muscle satellite cells
muscle stem cells
myogenesis
myogenic precursors
myogenic progenitors
postnatal growth
quiescence
Journal
Stem cell reports
ISSN: 2213-6711
Titre abrégé: Stem Cell Reports
Pays: United States
ID NLM: 101611300
Informations de publication
Date de publication:
08 09 2020
08 09 2020
Historique:
received:
13
02
2020
revised:
09
07
2020
accepted:
10
07
2020
pubmed:
9
8
2020
medline:
2
7
2021
entrez:
9
8
2020
Statut:
ppublish
Résumé
Muscle stem cells (or muscle satellite cells [MuSCs]) are required for postnatal growth. Yet, the detailed characterization of myogenic progression and establishment of quiescence during this process remains poorly documented. Here, we provide an overview of myogenic cells heterogeneity and dynamic from birth to adulthood using flow cytometry. We demonstrated that PAX7+ cells acquire an increasing ability to progress in the myogenic program from birth to adulthood. We then simultaneously analyzed the cycling state (KI67 expression) of the MuSCs and progenitors (PAX7+) and their progression into myogenic precursors (PAX7-MYOD+) and differentiating cells (MYOG+) in vivo. We identified two distinct peaks of myogenic differentiation between P7-P10 and P21-P28, and showed that the quiescent MuSC pool is established between 7 and 8 weeks of age. Overall our study provides a comprehensive in vivo characterization of myogenic heterogeneity and demonstrates the highly dynamic nature of skeletal muscle postnatal growth process.
Identifiants
pubmed: 32763161
pii: S2213-6711(20)30288-5
doi: 10.1016/j.stemcr.2020.07.011
pmc: PMC7486220
pii:
doi:
Substances chimiques
Antigens, CD
0
Antigens, CD34
0
Integrin alpha Chains
0
integrin alpha7
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
597-611Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
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