Modulation of glycosyltransferase ST6Gal-I in gastric cancer-derived organoids disrupts homeostatic epithelial cell turnover.
Antigens, CD
/ biosynthesis
Cell Line
Epithelial Cells
/ metabolism
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Neoplastic
Homeostasis
Humans
Neoplasm Proteins
/ biosynthesis
Neoplastic Stem Cells
/ metabolism
Organoids
/ metabolism
SOX9 Transcription Factor
/ genetics
Sialyltransferases
/ biosynthesis
Stomach Neoplasms
/ epidemiology
Tumor Cells, Cultured
apoptosis
cancer biology
epithelial cell
gastric cancer
glycosylation
sialic acid
stem cells
stem cellsα-26-sialyltransferase 1 (ST6Gal-I)
β-galactoside α2,6-sialyltransferase 1 (ST6GAL1)
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
09 10 2020
09 10 2020
Historique:
received:
17
06
2020
revised:
05
08
2020
pubmed:
9
8
2020
medline:
17
2
2021
entrez:
9
8
2020
Statut:
ppublish
Résumé
Programmed cell death promotes homeostatic cell turnover in the epithelium but is dysregulated in cancer. The glycosyltransferase ST6Gal-I is known to block homeostatic apoptosis through α2,6-linked sialylation of the death receptor TNFR1 in many cell types. However, its role has not been investigated in gastric epithelial cells or gastric tumorigenesis. We determined that human gastric antral epithelium rarely expressed ST6Gal-I, but the number of ST6Gal-I-expressing epithelial cells increased significantly with advancing premalignancy leading to cancer. The mRNA expression levels of
Identifiants
pubmed: 32763973
pii: S0021-9258(17)49810-3
doi: 10.1074/jbc.RA120.014887
pmc: PMC7549044
doi:
Substances chimiques
Antigens, CD
0
Neoplasm Proteins
0
SOX9 Transcription Factor
0
SOX9 protein, human
0
Sialyltransferases
EC 2.4.99.-
ST6GAL1 protein, human
EC 2.4.99.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
14153-14163Subventions
Organisme : NIAID NIH HHS
ID : T32 AI007051
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA225177
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA233581
Pays : United States
Organisme : NICHD NIH HHS
ID : R21 HD088954
Pays : United States
Organisme : NCRR NIH HHS
ID : C06 RR020136
Pays : United States
Informations de copyright
© 2020 Alexander et al.
Déclaration de conflit d'intérêts
Conflict of interest—The authors declare that they have no conflicts of interest with the contents of this article.
Références
Gastroenterology. 2015 Oct;149(4):918-27.e6
pubmed: 26116801
Nat Protoc. 2018 Feb;13(2):217-234
pubmed: 29300388
J Cell Biochem. 2008 Jul 1;104(4):1124-49
pubmed: 18459149
J Biol Chem. 2011 Nov 11;286(45):39654-62
pubmed: 21930713
Nature. 2017 Aug 24;548(7668):451-455
pubmed: 28813421
Pediatr Blood Cancer. 2011 Sep;57(3):524-7
pubmed: 21744476
Nature. 2016 Feb 18;530(7590):340-3
pubmed: 26863187
Cell Death Differ. 2003 Jan;10(1):45-65
pubmed: 12655295
Science. 2012 Oct 5;338(6103):108-13
pubmed: 22956684
J Immunol. 1999 Jan 15;162(2):1042-8
pubmed: 9916731
Exp Biol Med (Maywood). 2017 Oct;242(16):1633-1642
pubmed: 28534432
J Oncol. 2019 Apr 1;2019:6754040
pubmed: 31057614
Nat Protoc. 2013 Dec;8(12):2471-82
pubmed: 24232249
J Biochem. 1979 Nov;86(5):1573-85
pubmed: 42648
J Biol Chem. 2002 Sep 6;277(36):32930-8
pubmed: 12068010
J Natl Compr Canc Netw. 2015 Apr;13(4):e19-29
pubmed: 26052595
Stem Cell Res. 2019 May;37:101430
pubmed: 30933720
Trends Cell Biol. 2013 Dec;23(12):620-33
pubmed: 23958396
Nat Rev Mol Cell Biol. 2008 Aug;9(8):655-62
pubmed: 18545270
Mol Cell Proteomics. 2012 Dec;11(12):1913-23
pubmed: 23023295
Eur J Cancer. 2014 May;50(7):1330-44
pubmed: 24650579
Oncotarget. 2017 Nov 6;8(68):113163-113173
pubmed: 29348895
Int J Cancer. 2004 Mar;109(1):138-43
pubmed: 14735480
Cancer Metastasis Rev. 2012 Dec;31(3-4):501-18
pubmed: 22699311
Proc Natl Acad Sci U S A. 1999 Jul 20;96(15):8359-64
pubmed: 10411880
Cell. 2003 Jul 25;114(2):181-90
pubmed: 12887920
J Pediatr Surg. 2004 Aug;39(8):e8-10
pubmed: 15300556
J Biol Chem. 2018 Jan 19;293(3):984-994
pubmed: 29191829
Cell. 2018 Sep 6;174(6):1586-1598.e12
pubmed: 30100188
Nat Rev Microbiol. 2013 Jun;11(6):385-99
pubmed: 23652324
BMC Cancer. 2009 Dec 10;9:431
pubmed: 20003255
Gastroenterology. 2007 Aug;133(2):659-72
pubmed: 17681184
Science. 2014 Aug 22;345(6199):1247391
pubmed: 25146295
Cancer Res. 2016 Jul 1;76(13):3978-88
pubmed: 27216178
Gastroenterology. 2017 Aug;153(2):420-429
pubmed: 28456631
Gastroenterology. 2008 Feb;134(2):491-9
pubmed: 18242215
Immunity. 2004 Sep;21(3):415-28
pubmed: 15357952
Cell. 2000 Jan 7;100(1):57-70
pubmed: 10647931
Cytokine Growth Factor Rev. 2016 Dec;32:63-73
pubmed: 27461871
Cancer Res. 2012 Mar 1;72(5):1301-15
pubmed: 22246670
Nature. 2001 May 17;411(6835):342-8
pubmed: 11357141
J Biol Chem. 1999 Apr 9;274(15):10203-12
pubmed: 10187805
Int J Mol Sci. 2013 Jul 11;14(7):14475-503
pubmed: 23852022
J Biol Chem. 2018 Feb 2;293(5):1610-1622
pubmed: 29233887
J Biol Chem. 2011 Jul 1;286(26):22982-90
pubmed: 21550977
Infect Immun. 2015 Dec 07;84(2):562-72
pubmed: 26644382
Int J Cancer. 2006 Jun 15;118(12):3030-44
pubmed: 16404738
Am J Pathol. 1995 Jan;146(1):27-32
pubmed: 7856733
Int J Oncol. 2014 May;44(5):1433-42
pubmed: 24589677
CA Cancer J Clin. 2018 Nov;68(6):394-424
pubmed: 30207593
Clin Gastroenterol Hepatol. 2017 Dec;15(12):1833-1843
pubmed: 28532700
BMC Gastroenterol. 2017 Jun 29;17(1):84
pubmed: 28662697
Oncology. 2005;69(5):436-44
pubmed: 16319516
Cancer Res. 2013 Apr 1;73(7):2368-78
pubmed: 23358684
Gastroenterology. 1977 May;72(5 Pt 1):962-75
pubmed: 191330
Gut. 2015 Jun;64(6):911-20
pubmed: 25007816
J Clin Invest. 2004 Feb;113(3):321-33
pubmed: 14755326
Cell Stem Cell. 2010 Jan 8;6(1):25-36
pubmed: 20085740