Phenotypic and Functional Characterization of NK Cells in αβT-Cell and B-Cell Depleted Haplo-HSCT to Cure Pediatric Patients with Acute Leukemia.

NK alloreactivity NK cells haploidentical hematopoietic stem cell transplantation (HSCT) human cytomegalovirus (HCMV) killer Ig-like receptors (KIR) pediatric acute leukemia

Journal

Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829

Informations de publication

Date de publication:
05 Aug 2020
Historique:
received: 26 06 2020
revised: 31 07 2020
accepted: 01 08 2020
entrez: 9 8 2020
pubmed: 9 8 2020
medline: 9 8 2020
Statut: epublish

Résumé

NK cells can exert remarkable graft-versus-leukemia (GvL) effect in HLA-haploidentical hematopoietic stem cell transplantation (haplo-HSCT). Here, we dissected the NK-cell repertoire of 80 pediatric acute leukemia patients previously reported to have an excellent clinical outcome after αβT/B-depleted haplo-HSCT. This graft manipulation strategy allows the co-infusion of mature immune cells, mainly NK and γδT cells, and hematopoietic stem cells (HSCs). To promote NK-cell based antileukemia activity, 36/80 patients were transplanted with an NK alloreactive donor, defined according to the KIR/KIR-Ligand mismatch in the graft-versus-host direction. The analysis of the reconstituted NK-cell repertoire in these patients showed relatively high proportions of mature and functional KIR

Identifiants

pubmed: 32764469
pii: cancers12082187
doi: 10.3390/cancers12082187
pmc: PMC7463940
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : Associazione Italiana per la Ricerca sul Cancro
ID : 16764
Organisme : Associazione Italiana per la Ricerca sul Cancro
ID : 19920
Organisme : Associazione Italiana per la Ricerca sul Cancro
ID : 20312
Organisme : Associazione Italiana per la Ricerca sul Cancro
ID : 21724
Organisme : Associazione Italiana per la Ricerca sul Cancro
ID : 21147
Organisme : Accelerator Award 2017
ID : INCAR
Organisme : Ministero della Salute
ID : RF-2016-02364388
Organisme : Ministero della Salute
ID : 5xmille 2015 and 2016
Organisme : Ministero dell'Istruzione, dell'Università e della Ricerca
ID : PRIN 2017WC8499_004
Organisme : Horizon 2020
ID : MSCA-ITN-2017-765104-MATURE-NK
Organisme : Ministero della Salute
ID : RC-2019 IRCCS Ospedale Policlinico San Martino
Organisme : Ministero della Salute
ID : RC-2020 OPBG

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Auteurs

Raffaella Meazza (R)

Laboratory of Immunology, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Ospedale Policlinico San Martino, 16132 Genoa, Italy.

Michela Falco (M)

Laboratory of Clinical and Experimental Immunology, Integrated Department of Services and Laboratories, IRCCS Istituto Giannina Gaslini, 16147 Genoa, Italy.

Fabrizio Loiacono (F)

Laboratory of Immunology, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Ospedale Policlinico San Martino, 16132 Genoa, Italy.

Paolo Canevali (P)

Laboratory of Immunology, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Ospedale Policlinico San Martino, 16132 Genoa, Italy.

Mariella Della Chiesa (M)

Department of Experimental Medicine, Centre of Excellence for Biomedical Research, University of Genoa, 16132 Genoa, Italy.

Alice Bertaina (A)

Department of Hematology/Oncology, IRCCS Ospedale Pediatrico Bambino Gesù, 00146 Rome, Italy.

Daria Pagliara (D)

Department of Hematology/Oncology, IRCCS Ospedale Pediatrico Bambino Gesù, 00146 Rome, Italy.

Pietro Merli (P)

Department of Hematology/Oncology, IRCCS Ospedale Pediatrico Bambino Gesù, 00146 Rome, Italy.

Valentina Indio (V)

Giorgio Prodi Interdepartmental Center for Cancer Research-CIRC, University of Bologna, 40138 Bologna, Italy.

Federica Galaverna (F)

Department of Hematology/Oncology, IRCCS Ospedale Pediatrico Bambino Gesù, 00146 Rome, Italy.

Mattia Algeri (M)

Department of Hematology/Oncology, IRCCS Ospedale Pediatrico Bambino Gesù, 00146 Rome, Italy.

Francesca Moretta (F)

Department of Hematology/Oncology, IRCCS Ospedale Pediatrico Bambino Gesù, 00146 Rome, Italy.

Natalia Colomar-Carando (N)

Laboratory of Immunology, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Ospedale Policlinico San Martino, 16132 Genoa, Italy.
Department of Experimental Medicine, Centre of Excellence for Biomedical Research, University of Genoa, 16132 Genoa, Italy.

Letizia Muccio (L)

Department of Experimental Medicine, University of Genoa, 16132 Genoa, Italy.

Simona Sivori (S)

Department of Experimental Medicine, Centre of Excellence for Biomedical Research, University of Genoa, 16132 Genoa, Italy.

Andrea Pession (A)

Giorgio Prodi Interdepartmental Center for Cancer Research-CIRC, University of Bologna, 40138 Bologna, Italy.
Department of Pediatrics, University of Bologna, 40138 Bologna, Italy.

Maria Cristina Mingari (MC)

Laboratory of Immunology, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Ospedale Policlinico San Martino, 16132 Genoa, Italy.
Department of Experimental Medicine, Centre of Excellence for Biomedical Research, University of Genoa, 16132 Genoa, Italy.

Lorenzo Moretta (L)

Department of Immunology, IRCCS Ospedale Pediatrico Bambino Gesù, 00146 Rome, Italy.

Alessandro Moretta (A)

Department of Experimental Medicine, Centre of Excellence for Biomedical Research, University of Genoa, 16132 Genoa, Italy.

Franco Locatelli (F)

Department of Hematology/Oncology, IRCCS Ospedale Pediatrico Bambino Gesù, 00146 Rome, Italy.
Department of Gynecology/Obstetrics and Pediatrics, Sapienza University, 00185 Rome, Italy.

Daniela Pende (D)

Laboratory of Immunology, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Ospedale Policlinico San Martino, 16132 Genoa, Italy.

Classifications MeSH