From 2D to 3D: Promising Advances in Imaging Lung Structure.
STED
confocal
lung structure
optical clearing
precision cut lung slices
vibratome
Journal
Frontiers in medicine
ISSN: 2296-858X
Titre abrégé: Front Med (Lausanne)
Pays: Switzerland
ID NLM: 101648047
Informations de publication
Date de publication:
2020
2020
Historique:
received:
30
03
2020
accepted:
09
06
2020
entrez:
9
8
2020
pubmed:
9
8
2020
medline:
9
8
2020
Statut:
epublish
Résumé
The delicate structure of murine lungs poses many challenges for acquiring high-quality images that truly represent the living lung. Here, we describe several optimized procedures for obtaining and imaging murine lung tissue. Compared to traditional paraffin cross-section and optimal cutting temperature (OCT), agarose-inflated vibratome sections (aka precision-cut lung slices), combines comparable structural preservation with experimental flexibility. In particular, we discuss an optimized procedure to precision-cut lung slices that can be used to visualize three-dimensional cell-cell interactions beyond the limitations of two-dimensional imaging. Super-resolution microscopy can then be used to reveal the fine structure of lung tissue's cellular bodies and processes that regular confocal cannot. Lastly, we evaluate the entire lung vasculature with clearing technology that allows imaging of the entire volume of the lung without sectioning. In this manuscript, we combine the above procedures to create a novel and evolutionary method to study cell behavior
Identifiants
pubmed: 32766264
doi: 10.3389/fmed.2020.00343
pmc: PMC7381109
doi:
Types de publication
Journal Article
Langues
eng
Pagination
343Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL095686
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL141105
Pays : United States
Informations de copyright
Copyright © 2020 Klouda, Condon, Hao, Tian, Lvova, Chakraborty, Nicolls, Zhou, Raby and Yuan.
Références
Sci Rep. 2018 Sep 6;8(1):13348
pubmed: 30190498
Antiviral Res. 2015 Aug;120:101-11
pubmed: 26022197
J Clin Invest. 2012 Dec;122(12):4439-46
pubmed: 23143304
Mol Ther Methods Clin Dev. 2018 Aug 04;10:245-256
pubmed: 30112421
Sci Adv. 2019 Jan 11;5(1):eaau8355
pubmed: 30746463
Proc Natl Acad Sci U S A. 2008 Dec 2;105(48):18982-7
pubmed: 19028874
Am J Respir Cell Mol Biol. 2014 May;50(5):876-81
pubmed: 24313705
Paediatr Respir Rev. 2017 Jun;23:40-49
pubmed: 28416135
Nat Commun. 2017 Jul 25;8(1):127
pubmed: 28743899
Angiogenesis. 2017 Nov;20(4):533-546
pubmed: 28699046
PLoS One. 2014 Jun 09;9(6):e96551
pubmed: 24911061
Bio Protoc. 2017 Sep 5;7(17):
pubmed: 29057295
Pulm Pharmacol Ther. 2011 Oct;24(5):452-65
pubmed: 21600999
BMC Neurosci. 2011 Jan 27;12:16
pubmed: 21272290
mBio. 2020 Feb 4;11(1):
pubmed: 32019790
Am J Respir Cell Mol Biol. 2020 Jun;62(6):747-759
pubmed: 32084325
Nat Protoc. 2014 Jul;9(7):1682-97
pubmed: 24945384
Cell. 2015 Jul 16;162(2):246-257
pubmed: 26186186
Am J Respir Cell Mol Biol. 2017 Jun;56(6):694-699
pubmed: 28080134
Br J Pharmacol. 1999 Mar;126(5):1191-9
pubmed: 10205008
Nat Protoc. 2010 Sep;5(9):1540-51
pubmed: 20725069
Respir Res. 2019 Jul 19;20(1):162
pubmed: 31324219
Nat Neurosci. 2019 Feb;22(2):317-327
pubmed: 30598527
Sci Immunol. 2018 Jan 12;3(19):
pubmed: 29330161
J Intern Med. 2014 Dec;276(6):560-78
pubmed: 24980774
Elife. 2015 Oct 19;4:
pubmed: 26479710
Blood. 2018 Apr 19;131(16):1858-1869
pubmed: 29463561
J Invest Dermatol. 2012 Dec;132(12):e3
pubmed: 23187113
Annu Rev Gerontol Geriatr. 1986;6:197-214
pubmed: 3113451