Basophil Activation Techniques: Staining of Exteriorized Granule Matrix.
Avidin
/ chemistry
Basophils
/ cytology
Biomarkers
/ analysis
Cell Degranulation
Cells, Cultured
Extracellular Space
/ chemistry
Flow Cytometry
/ methods
Fluoresceins
/ chemistry
Humans
Immunophenotyping
/ methods
Proteoglycans
/ analysis
Secretory Vesicles
/ chemistry
Staining and Labeling
/ methods
Basophil activation test (BAT)
Basophils
CD203c
Granule matrix
Proteoglycans
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
9
8
2020
pubmed:
9
8
2020
medline:
13
3
2021
Statut:
ppublish
Résumé
The basis of traditional flow cytometry allergy diagnosis is measurement of the expression of basophilic surface activation and/or degranulation markers. Basophils, upon encounter with a specific allergen that cross-links surface FcRI-bound IgE antibodies, not only secrete and release quantifiable bioactive mediators but also upregulate the expression of different markers (e.g., CD63, CD203c) which can be detected by multicolor flow cytometry using specific monoclonal antibodies. Here, we describe a novel technique that relies upon the staining of exteriorized anionic proteoglycans from a basophil granule matrix by cationic fluorescent avidin probes.
Identifiants
pubmed: 32766978
doi: 10.1007/978-1-0716-0696-4_17
doi:
Substances chimiques
Biomarkers
0
Fluoresceins
0
Proteoglycans
0
fluorescein-avidin
0
Avidin
1405-69-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
213-218Références
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pubmed: 1716273
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doi: 10.1016/j.jaci.2017.03.030
pubmed: 28433691
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doi: 10.1016/j.jaci.2017.03.052
pubmed: 28606590
pmcid: 5632583