Measurement and Functional Analysis of the Mas-Related G Protein-Coupled Receptor MRGPRX2 on Human Mast Cells and Basophils.
Basophils
Cultured mast cells
Flow cytometry
MRGPRX2
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
9
8
2020
pubmed:
9
8
2020
medline:
13
3
2021
Statut:
ppublish
Résumé
Basophils and mast cells (MCs) are important effector cells in the immune system. For a long time, it has been known that these cells can be activated though the cross-linking of IgE antibodies bound to their high-affinity receptor (FcεRI). However, evidence has accumulated suggesting that these cells can also be activated by various IgE-independent mechanisms. Occupation of MAS Related GPR Family Member X2 (MRGPRX2), a G protein-coupled receptor, is described as an alternative IgE-independent activation mechanism. Here we describe a flow cytometric technique to analyze MRGPRX2 expression and its functionality on cultured human MCs and conditioned basophils, that is, basophils with upregulated surface expression of MRGPRX2.
Identifiants
pubmed: 32766979
doi: 10.1007/978-1-0716-0696-4_18
doi:
Substances chimiques
Biomarkers
0
MRGPRX2 protein, human
0
Nerve Tissue Proteins
0
Receptors, G-Protein-Coupled
0
Receptors, Neuropeptide
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
219-226Références
Decuyper II, Mangodt EA, Van Gasse AL, Claesen K, Uyttebroek A, Faber M et al (2017) In vitro diagnosis of immediate drug hypersensitivity anno 2017: potentials and limitations. Drugs R D 17(2):265–278. https://doi.org/10.1007/s40268-017-0176-x
doi: 10.1007/s40268-017-0176-x
pubmed: 28258478
pmcid: 5427047
Gaudenzio N, Sibilano R, Marichal T, Starkl P, Reber LL, Cenac N et al (2016) Different activation signals induce distinct mast cell degranulation strategies. J Clin Invest 126(10):3981–3998. https://doi.org/10.1172/JCI85538
doi: 10.1172/JCI85538
pubmed: 27643442
pmcid: 5096814
McNeil BD, Pundir P, Meeker S, Han L, Undem BJ, Kulka M et al (2015) Identification of a mast-cell-specific receptor crucial for pseudo-allergic drug reactions. Nature 519:237–241. https://doi.org/10.1038/nature14022
doi: 10.1038/nature14022
pubmed: 25517090
Sabato V, Van Gasse A, Cop N et al (2017) The Mas-related G protein-coupled receptor MRGPRX2 is expressed on human basophils and up-regulated upon activation. J Allergy Clin Immun 139(2):Ab168-Ab. https://doi.org/10.1016/j.jaci.2016.12.550
doi: 10.1016/j.jaci.2016.12.550
Cop N, Decuyper II, Faber MA, Sabato V, Bridts CH, Hagendorens MM et al (2017) Phenotypic and functional characterization of in vitro cultured human mast cells. Cytometry B Clin Cytom 92(5):348–354. https://doi.org/10.1002/cyto.b.21399
doi: 10.1002/cyto.b.21399
pubmed: 27401129