Regulation of photosystem I-light-harvesting complex I from a red alga Cyanidioschyzon merolae in response to light intensities.
Cyanidioschyzon merolae
Fluorescence decay
Light adaptation
Light-harvesting antenna
PSI-LHCI
Red algae
Journal
Photosynthesis research
ISSN: 1573-5079
Titre abrégé: Photosynth Res
Pays: Netherlands
ID NLM: 100954728
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
09
04
2020
accepted:
21
07
2020
pubmed:
9
8
2020
medline:
3
8
2021
entrez:
9
8
2020
Statut:
ppublish
Résumé
Photosynthetic organisms use different means to regulate their photosynthetic activity in respond to different light conditions under which they grow. In this study, we analyzed changes in the photosystem I (PSI) light-harvesting complex I (LHCI) supercomplex from a red alga Cyanidioschyzon merolae, upon growing under three different light intensities, low light (LL), medium light (ML), and high light (HL). The results showed that the red algal PSI-LHCI is separated into two bands on blue-native PAGE, which are designated PSI-LHCI-A and PSI-LHCI-B, respectively, from cells grown under LL and ML. The former has a higher molecular weight and binds more Lhcr subunits than the latter. They are considered to correspond to the two types of PSI-LHCI identified by cryo-electron microscopic analysis recently, namely, the former with five Lhcrs and the latter with three Lhcrs. The amount of PSI-LHCI-A is higher in the LL-grown cells than that in the ML-grown cells. In the HL-grown cells, PSI-LHCI-A completely disappeared and only PSI-LHCI-B was observed. Furthermore, PSI core complexes without Lhcr attached also appeared in the HL cells. Fluorescence decay kinetics measurement showed that Lhcrs are functionally connected with the PSI core in both PSI-LHCI-A and PSI-LHCI-B obtained from LL and ML cells; however, Lhcrs in the PSI-LHCI-B fraction from the HL cells are not coupled with the PSI core. These results indicate that the red algal PSI not only regulates its antenna size but also adjusts the functional connection of Lhcrs with the PSI core in response to different light intensities.
Identifiants
pubmed: 32766997
doi: 10.1007/s11120-020-00778-z
pii: 10.1007/s11120-020-00778-z
doi:
Substances chimiques
Light-Harvesting Protein Complexes
0
Photosystem I Protein Complex
0
Chlorophyll
1406-65-1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
287-297Subventions
Organisme : Key Technology Research and Development Program of Shandong
ID : 2017YFA0503700
Organisme : National Natural Science Foundation of China
ID : 31470339
Organisme : Strategic Priority Research Program of Chinese Academy of Sciences
ID : XDB17000000
Organisme : Key Research Program of Frontier Sciences, CAS
ID : QYZDY-SSW-SMC003
Organisme : Natural Science Foundation of Hebei Province (CN)
ID : C2020205051
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