Medicinal plant extracts protect epithelial cells from infection and DNA damage caused by colibactin-producing Escherichia coli, and inhibit the growth of bacteria.
Animals
Anti-Bacterial Agents
/ pharmacology
Caco-2 Cells
Chlorocebus aethiops
DNA Damage
Epithelial Cells
/ drug effects
Escherichia coli
/ drug effects
Escherichia coli Proteins
/ genetics
Gene Expression Regulation, Bacterial
/ drug effects
Humans
Meliaceae
/ chemistry
Peptides
/ genetics
Plant Extracts
/ pharmacology
Plants, Medicinal
/ chemistry
Polyketides
/ metabolism
Psidium
/ chemistry
Terminalia
/ chemistry
Thailand
Vero Cells
Escherichia coli
DNA damage
anti-bacteria
colibactin
medicinal plant
transient infection
Journal
Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
23
02
2020
revised:
28
07
2020
accepted:
03
08
2020
pubmed:
9
8
2020
medline:
22
4
2021
entrez:
9
8
2020
Statut:
ppublish
Résumé
To investigate the biological activity of Thai medicinal plant extracts and their active substances on the inhibition of growth and the transcription of colibactin genes of colibactin-producing Escherichia coli, and effect on the pathogenesis from colibactin toxin including transient infections and colibactin-induced DNA damage. Among 16 medicinal plants examined, aqueous extracts of Terminalia catappa, Psidium guajava and Sandoricum koetjape demonstrated the growth inhibition against E. coli ATCC 25922, which is known to produce colibactin toxin. These plant extracts contain the active phytochemical compounds, tannin and quercetin, which are able to inhibit the growth of E. coli ATCC 25922. Interestingly, the extracts of T. catappa, P. guajava and S. koetjape, and their compounds tannin and quercetin, protected the eukaryotic epithelial cells of Vero cells and Caco-2 cells from infection and DNA damage by E. coli ATCC 25922. Moreover, these plant extracts and compounds exhibited efficacy to downregulate the expression of five genes (clbA, clbB, clbM, clbN and clbP) that are required for colibactin biosynthesis. The extracts of T. catappa, P. guajava and S. koetjape, and their compounds of tannin and quercetin had ability to inhibit the growth and transcription of colibactin genes of colibactin-producing Escherichia coli. Hence, these plant extracts and compounds could protect the transient infection and DNA damage of the eukaryotic epithelial cells. This study is the first of its kind to report on the enhancement of the biological properties of T. catappa, P. guajava and S. koetjape, and to support the exogenous compound usage of tannin and quercetin, which may be able to protect against the transient infection and DNA damage of eukaryotic cells from E. coli carrying colibactin toxin.
Substances chimiques
Anti-Bacterial Agents
0
Escherichia coli Proteins
0
Peptides
0
Plant Extracts
0
Polyketides
0
colibactin
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
769-785Subventions
Organisme : Thailand Research Fund
ID : PHD/0061/2558
Organisme : Thailand Research Fund
ID : PHD/0061/2558;
Informations de copyright
© 2020 The Society for Applied Microbiology.
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