Mineral trioxide aggregate suppresses pro-inflammatory cytokine expression via the calcineurin/nuclear factor of activated T cells/early growth response 2 pathway in lipopolysaccharide-stimulated macrophages.
M2 macrophage
calcineurin
calcium ion
early growth response 2
mineral trioxide aggregate
nuclear factor of activated T cells
Journal
International endodontic journal
ISSN: 1365-2591
Titre abrégé: Int Endod J
Pays: England
ID NLM: 8004996
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
30
03
2020
revised:
27
06
2020
accepted:
04
08
2020
pubmed:
9
8
2020
medline:
15
12
2020
entrez:
9
8
2020
Statut:
ppublish
Résumé
To elucidate mechanisms by which mineral trioxide aggregate (MTA) suppresses pro-inflammatory cytokine mRNA expression in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Mineral trioxide aggregate extracts were prepared by immersing set ProRoot MTA in culture medium. RAW264.7 cells were cultured in the presence of LPS and MTA extracts. mRNA expression levels of interleukin (IL)-1α, IL-6, early growth response 2 (Egr2), suppressor of cytokine signalling 3 (Socs3) and IL-10 were quantified with reverse transcription-quantitative polymerase chain reaction. Phosphorylation of nuclear factor-kappa B (NF-κB) p65 in RAW264.7 cells was analysed by Western blotting. Intracellular calcium imaging was performed with Fluo-4 AM. The activity of nuclear factor of activated T cells (NFAT) was determined by luciferase assays. Enforced expression and silencing of Egr2 in RAW264.7 cells were carried out using an expression vector and specific RNAi, respectively. In vivo kinetics of Egr2 Exposure to MTA extracts resulted in reduced mRNA expression levels of IL-1α and IL-6, as well as reduced expression of phosphorylated NF-κB, in LPS-stimulated RAW264.7 cells. Exposure to MTA extracts induced Ca Mineral trioxide aggregate extracts induced downregulation of IL-1α and IL-6 in LPS-stimulated RAW264.7 cells via CaSR-induced activation of calcineurin/NFAT/Egr2 signalling and subsequent upregulation of IL-10 and Socs3.
Substances chimiques
Aluminum Compounds
0
Calcium Compounds
0
Cytokines
0
Drug Combinations
0
Lipopolysaccharides
0
NF-kappa B
0
Oxides
0
Silicates
0
mineral trioxide aggregate
0
Calcineurin
EC 3.1.3.16
Types de publication
Journal Article
Langues
eng
Pagination
1653-1665Subventions
Organisme : Japan Society for the Promotion of Science
ID : 16K15795
Organisme : Japan Society for the Promotion of Science
ID : 19K24136
Organisme : Japan Society for the Promotion of Science
ID : 19K24113
Organisme : Japan Society for the Promotion of Science
ID : 19K24137
Informations de copyright
© 2020 International Endodontic Journal. Published by John Wiley & Sons Ltd.
Références
Carow B, Rottenberg ME (2020) SOCS3, a major regulator of infection and inflammation. Frontiers in Immunology 5, 1-13.
Cavenago BC, Pereira TC, Duarte MAH et al. (2014) Influence of powder-to-water ratio on radiopacity, setting time, pH, calcium ion release and a micro-CT volumetric solubility of white mineral trioxide aggregate. International Endodontic Journal 47, 120-6.
Chang J, Kunkel SL, Chang CH (2009) Negative regulation of MyD88-dependent signaling by IL-10 in dendritic cells. Proceedings of the National Academy of Sciences of the USA 106, 18327-32.
Chong BS, Pitt Ford TR, Hudson MB (2009) A prospective clinical study of mineral trioxide aggregate and IRM when used as root-end filling materials in endodontic surgery. International Endodontic Journal 42, 414-20.
Clapham DE (2007) Calcium signaling. Cell 131, 1047-58.
Clipstone NA, Crabtree GR (1992) Identification of calcineurin as a key signalling enzyme in T-lymphocyte activation. Nature 357, 695-7.
Couper KN, Blount DG, Riley EM (2008) IL-10: the master regulator of immunity to infection. Journal of Immunology 180, 5771-7.
Crabtree GR, Olson EN (2002) NFAT signaling: choreographing the social lives of cells. Cell 109, S67-S79.
Fabriek BO, Dijkstra CD, van den Berg TK (2005) The macrophage scavenger receptor CD163. Immunobiology 210, 153-60.
Goldberg M, Njeh A, Uzunoglu E (2015) Is Pulp Inflammation a Prerequisite for Pulp Healing and Regeneration? Mediators of Inflammation, 2015 1-11. http://dx.doi.org/10.1155/2015/347649
Gordon S (2003) Alternative activation of macrophages. Nature Reviews Immunology 3, 23-35.
Harris JE, Bishop KD, Phillips NE et al. (2004) Early growth response gene-2, a zinc-finger transcription factor, is required for full induction of clonal anergy in CD4 + T Cells. Journal of Immunology 173, 7331-8.
Hashimoto K, Kawashima N, Ichinose S, Nara K, Noda S, Okiji T (2018) EDTA treatment for sodium hypochlorite-treated dentin recovers disturbed attachment and induces differentiation of mouse dental papilla cells. Journal of Endodontics 44, 256-62.
Hofer AM, Brown EM (2003) Extracellular calcium sensing and signalling. Nature Reviews Molecular Cell Biology 4, 530-8.
Holland R, Arlindo Otoboni Filho J, de Souza V, Juvenal Nery M, Felício Estrada Bernabé P, Dezan Junior E (2001) Mineral trioxide aggregate repair of lateral root perforations. Journal of Endodontics 27, 281-4.
Italiani P, Boraschi D (2014) From monocytes to M1/M2 macrophages: phenotypical vs. functional differentiation. Frontiers in Immunology 5, 1-22.
Ito T, Kaneko T, Yamanaka Y, Shigetani Y, Yoshiba K, Okiji T (2014) M2 macrophages participate in the biological tissue healing reaction to mineral trioxide aggregate. Journal of Endodontics 40, 379-83.
Jablonski KA, Amici SA, Webb LM et al. (2015) Novel markers to delineate murine M1 and M2 macrophages. PLoS One 10, 5-11.
Josefowicz SZ, Rudensky A (2009) Control of regulatory T cell lineage commitment and maintenance. Immunity 30, 616-25.
Kawai T, Akira S (2007) Signaling to NF-κB by Toll-like receptors. Trends in Molecular Medicine 13, 460-9.
Kawashima N, Nakano-Kawanishi H, Suzuki N, Takagi M, Suda H (2005) Effect of NOS inhibitor on cytokine and COX2 expression in rat pulpitis. Journal of Dental Research 84, 762-7.
Kim DH, Jang JH, Lee BN et al. (2018) Anti-inflammatory and mineralization effects of proroot MTA and endocem MTA in studies of human and rat dental pulps in vitro and in vivo. Journal of Endodontics 44, 1534-41.
Kim JM, Choi S, Kwack KH, Kim SY, Lee HW, Park K (2017) G protein-coupled calcium-sensing receptor is a crucial mediator of MTA-induced biological activities. Biomaterials 127, 107-16.
Koh ET, Torabinejad M, Pitt Ford TR, Brady K, McDonald F (1997) Mineral trioxide aggregate stimulates a biological response in human osteoblasts. Journal of Biomedical Materials Research 37, 432-9.
Kundzina R, Stangvaltaite L, Eriksen HM, Kerosuo E (2017) Capping carious exposures in adults: a randomized controlled trial investigating mineral trioxide aggregate versus calcium hydroxide. International Endodontic Journal 50, 924-32.
Lai WY, Kao CT, Hung CJ, Huang TH, Shie MY (2014) An evaluation of the inflammatory response of lipopolysaccharide-treated primary dental pulp cells with regard to calcium silicate-based cements. International Journal of Oral Science 6, 94-8.
Lazarevic V, Zullo AJ, Schweitzer MN et al. (2009) The gene encoding early growth response 2, a target of the transcription factor NFAT, is required for the development and maturation of natural killer T cells. Nature Immunology 10, 306-13.
Lee BN, Hong JU, Kim SM et al. (2019) Anti-inflammatory and osteogenic effects of calcium silicate-based root canal sealers. Journal of Endodontics 45, 73-8.
Li S, Miao T, Sebastian M et al. (2012) The transcription factors Egr2 and Egr3 are esential for the control of inflammation and antigen-induced proliferation of B and T cells. Immunity 37, 685-96.
Mente J, Hufnagel S, Leo M et al. (2014) Treatment outcome of mineral trioxide aggregate or calcium hydroxide direct pulp capping: Long-term results. Journal of Endodontics 40, 1746-51.
Morita K, Okamura T, Sumitomo S, Iwasaki Y, Fujio K, Yamamoto K (2016) Emerging roles of Egr2 and Egr3 in the control of systemic autoimmunity. Rheumatology (Oxford) 55, ii76-81.
Mosser DM, Edwards JP (2008) Exploring the full spectrum of macrophage activation. Nature Reviews Immunology 8, 958-69.
Nowicka A, Lipski M, Parafiniuk M et al. (2013) Response of human dental pulp capped with biodentine and mineral trioxide aggregate. Journal of Endodontics 39, 743-7.
O’Donovan KJ, Tourtellotte WG, Millbrandt J, Baraban JM (1999) The EGR family of transcription-regulatory factors: progress at the interface of molecular and systems neuroscience. Trends in Neurosciences 22, 167-73.
Okamura T, Fujio K, Shibuya M et al. (2009) CD4+CD25-LAG3+ regulatory T cells controlled by the transcription factor Egr-2. Proceedings of the National Academy of Sciences of the USA, 106, 13974-9.
Okiji T, Kawashima N, Kosaka T, Matsumoto A, Kobayashi C, Suda H (1992) An immunohistochemical study of the distribution of immunocompetent cells, especially macrophages and Ia antigen-expressing cells of heterogeneous populations, in normal rat molar pulp. Journal of Dental Research 71, 1196-202.
Okiji T, Yoshiba K (2009) Reparative dentinogenesis induced by mineral trioxide aggregate: a review from the biological and physicochemical points of view. International Journal of Dentistry 2009, 1-12.
Parirokh M, Torabinejad M, Dummer PMH (2018) Mineral trioxide aggregate and other bioactive endodontic cements: an updated overview - part I: vital pulp therapy. International Endodontic Journal 51, 177-205.
Hogan PG, Chen L, Julie Nardone AR (2003) Transcriptional regulation by calcium, calcineurin and NFAT. Genes & Development 17, 2205-32.
Polfliet MMJ, Fabriek BO, Daniëls WP, Dijkstra CD, van den Berg TK (2006) The rat macrophage scavenger receptor CD163: Expression, regulation and role in inflammatory mediator production. Immunobiology 211, 419-25.
Primus CM, Tay FR, Niu L (2019) Bioactive tri/dicalcium silicate cements for treatment of pulpal and periapical tissues. Acta Biomaterialia 96, 35-54.
Reyes-Carmona JF, Santos AS, Figueiredo CP et al. (2010) Host-mineral trioxide aggregate inflammatory molecular signaling and biomineralization ability. Journal of Endodontics 36, 1347-53.
Rusnak F, Mertz P (2000) Calcineurin : form and function. Physiological Reviews 80, 1483-521.
Safford M, Collins S, Lutz MA et al. (2005) Egr-2 and Egr-3 are negative regulators of T cell activation. Nature Immunology 6, 472-80.
Schmitz J, Weissenbach M, Haan S, Heinrich PC, Schaper F (2000) SOCS3 exerts its inhibitory function on interleukin-6 signal transduction through the SHP2 recruitment site of gp130. Journal of Biological Chemistry 275, 12848-56.
Seki YI, Inoue H, Nagata N et al. (2003) SOCS-3 regulates onset and maintenance of T H 2-mediated allergic responses. Nature Medicine 9, 1047-54.
Seo M-S, Hwang K-G, Lee J, Kim H, Baek S-H (2013) The Effect of Mineral Trioxide Aggregate on Odontogenic Differentiation in Dental Pulp Stem Cells. Journal of Endodontics. http://dx.doi.org/10.1016/j.joen.2012.11.004
Serra MB, Barroso WA, Da SNN et al. (2017) From inflammation to current and alternative therapies involved in wound healing. International Journal of Inflammation 2017, 1-17.
Silva MJB, Vieira LQ, Sobrinho APR (2008) The effects of mineral trioxide aggregates on cytokine production by mouse pulp tissue. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology 105, e70-6.
Sridharan R, Cameron AR, Kelly DJ, Kearney CJ, O’Brien FJ (2015) Biomaterial based modulation of macrophage polarization: a review and suggested design principles. Materials Today 18, 313-25.
Taciak B, Białasek M, Braniewska A et al. (2018) Evaluation of phenotypic and functional stability of RAW 264.7 cell line through serial passages. PLoS One 13, 1-13.
Takei E, Shigetani Y, Yoshiba K, Hinata G, Yoshiba N, Okiji T (2014) Initial transient accumulation of M2 macrophage-associated molecule-expressing cells after pulpotomy with mineral trioxide aggregate in rat molars. Journal of Endodontics 40, 1983-8.
Torabinejad M, Watson TF, Pitt Ford TR (1993) Sealing ability of a mineral trioxide aggregate when used as a root end filling material. Journal of Endodontics 19, 591-5.
Wynn TA, Vannella KM (2016) Macrophages in tissue repair, regeneration, and fibrosis. Immunity 44, 450-62.
Yaemkleebbua K, Osathanon T, Nowwarote N, Limjeerajarus CN, Sukarawan W (2019) Analysis of hard tissue regeneration and Wnt signalling in dental pulp tissues after direct pulp capping with different materials. International Endodontic Journal 52, 1605-16.
Yamamoto S, Han L, Noiri Y, Okiji T (2017) Evaluation of the Ca ion release, pH and surface apatite formation of a prototype tricalcium silicate cement. International Endodontic Journal 50, e73-82.
Yeh HW, Chiang CF, Chen PH et al. (2018) Axl involved in mineral trioxide aggregate induces macrophage polarization. Journal of Endodontics 44, 1542-8.
Zhao X, He W, Song Z, Tong Z, Li S, Ni L (2012) Mineral trioxide aggregate promotes odontoblastic differentiation via mitogen-activated protein kinase pathway in human dental pulp stem cells. Molecular Biology Reports. http://dx.doi.org/10.1007/s11033-011-0728-z