Identification and characterization of novel small molecule inhibitors to control Mycoplasma gallisepticum infection in chickens.
Animals
Anti-Bacterial Agents
/ administration & dosage
Bacterial Outer Membrane
/ drug effects
Caco-2 Cells
Chickens
/ microbiology
Drug Resistance, Bacterial
Epithelial Cells
/ drug effects
Erythrocytes
/ drug effects
Humans
Macrophages
/ drug effects
Microbial Sensitivity Tests
Mycoplasma Infections
/ drug therapy
Mycoplasma gallisepticum
/ drug effects
Poultry Diseases
/ drug therapy
Respiratory Tract Infections
Small Molecule Libraries
/ pharmacology
Specific Pathogen-Free Organisms
Cell membrane
Chickens
Growth inhibitors
Microbiota
Mycoplasma gallisepticum
Small molecules
Journal
Veterinary microbiology
ISSN: 1873-2542
Titre abrégé: Vet Microbiol
Pays: Netherlands
ID NLM: 7705469
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
07
05
2020
revised:
09
07
2020
accepted:
11
07
2020
entrez:
10
8
2020
pubmed:
10
8
2020
medline:
15
5
2021
Statut:
ppublish
Résumé
Mycoplasma gallisepticum (MG) causes chronic respiratory disease in chickens, leading to severe economic losses to the poultry industry. Currently the disease is managed with antimicrobials and vaccination; however, emergence of multi-drug resistant Mycoplasma and the limited effect of vaccines necessitate development of novel approaches. A library of 4,182 small molecules (SMs) was screened for identification of narrow spectrum anti-MG compounds using high throughput screening. A total of 584 SMs were identified. Ten SMs possessed low MICs (0.78-100 μM) with efficacy against multiple MG strains and MG biofilm. These 10 SMs did not affect commensal/probiotic bacteria and other avian and foodborne pathogens. They displayed no or little toxicity on the avian macrophage HD-11 cells, human epithelial Caco-2 cells, and chicken red blood cells (RBCs); but, they were effective in reducing MG in chicken RBCs. Six SMs (SM1, SM3-5, and SM9-10) were tested in three-week-old chickens infected with MG (nasal spray; 10
Identifiants
pubmed: 32768201
pii: S0378-1135(20)30574-5
doi: 10.1016/j.vetmic.2020.108799
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Small Molecule Libraries
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108799Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare no conflict of interest.