STROBE-metagenomics: a STROBE extension statement to guide the reporting of metagenomics studies.
Journal
The Lancet. Infectious diseases
ISSN: 1474-4457
Titre abrégé: Lancet Infect Dis
Pays: United States
ID NLM: 101130150
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
13
12
2019
revised:
09
03
2020
accepted:
12
03
2020
pubmed:
10
8
2020
medline:
21
10
2020
entrez:
10
8
2020
Statut:
ppublish
Résumé
The term metagenomics refers to the use of sequencing methods to simultaneously identify genomic material from all organisms present in a sample, with the advantage of greater taxonomic resolution than culture or other methods. Applications include pathogen detection and discovery, species characterisation, antimicrobial resistance detection, virulence profiling, and study of the microbiome and microecological factors affecting health. However, metagenomics involves complex and multistep processes and there are important technical and methodological challenges that require careful consideration to support valid inference. We co-ordinated a multidisciplinary, international expert group to establish reporting guidelines that address specimen processing, nucleic acid extraction, sequencing platforms, bioinformatics considerations, quality assurance, limits of detection, power and sample size, confirmatory testing, causality criteria, cost, and ethical issues. The guidance recognises that metagenomics research requires pragmatism and caution in interpretation, and that this field is rapidly evolving.
Identifiants
pubmed: 32768390
pii: S1473-3099(20)30199-7
doi: 10.1016/S1473-3099(20)30199-7
pmc: PMC7406238
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
e251-e260Subventions
Organisme : Wellcome Trust
ID : 206478/Z/17/Z
Pays : United Kingdom
Organisme : NINDS NIH HHS
ID : NS003130
Pays : United States
Organisme : NINDS NIH HHS
ID : K08 NS096117
Pays : United States
Organisme : Wellcome Trust
ID : 204904/Z/16/Z
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
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