SenX3-RegX3, an Important Two-Component System, Regulates Strain Growth and Butenyl-spinosyn Biosynthesis in Saccharopolyspora pogona.
Genetics
Microbiology
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
21 Aug 2020
21 Aug 2020
Historique:
received:
04
12
2019
revised:
09
06
2020
accepted:
20
07
2020
pubmed:
10
8
2020
medline:
10
8
2020
entrez:
10
8
2020
Statut:
ppublish
Résumé
Butenyl-spinosyn produced by Saccharopolyspora pogona exhibits strong insecticidal activity and a broad pesticidal spectrum. Currently, important functional genes involved in butenyl-spinosyn biosynthesis remain unknown, which leads to difficulty in efficient understanding of its regulatory mechanism and improving its production by metabolic engineering. Here, we present data supporting a role of the SenX3-RegX3 system in regulating the butenyl-spinosyn biosynthesis. EMSAs and qRT-PCR demonstrated that RegX3 positively controls butenyl-spinosyn production in an indirect way. Integrated proteomic and metabolomic analysis, regX3 deletion not only strengthens the basal metabolic ability of S. pogona in the mid-growth phase but also promotes the flow of the acetyl-CoA produced via key metabolic pathways into the TCA cycle rather than the butenyl-spinosyn biosynthetic pathway, which ultimately leads to continued growth but reduced butenyl-spinosyn production. The strategy demonstrated here may be valuable for revealing the regulatory role of the SenX3-RegX3 system in the biosynthesis of other natural products.
Identifiants
pubmed: 32768668
pii: S2589-0042(20)30586-1
doi: 10.1016/j.isci.2020.101398
pmc: PMC7414002
pii:
doi:
Types de publication
Journal Article
Langues
eng
Pagination
101398Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare that they have no conflicts of interest.
Références
Proc Natl Acad Sci U S A. 2003 May 13;100(10):6133-8
pubmed: 12730372
Appl Microbiol Biotechnol. 2019 Feb;103(4):1643-1658
pubmed: 30627795
PLoS One. 2018 Dec 6;13(12):e0208278
pubmed: 30521601
Bioorg Med Chem. 2009 Jun 15;17(12):4185-96
pubmed: 19324553
J Bacteriol. 2007 Aug;189(15):5495-503
pubmed: 17526710
Appl Microbiol Biotechnol. 2015 Dec;99(24):10547-57
pubmed: 26298701
Appl Microbiol Biotechnol. 2018 Aug;102(16):7029-7045
pubmed: 29948118
Nucleic Acids Res. 2012 Oct;40(19):9543-56
pubmed: 22904076
Biotechnol J. 2016 Feb;11(2):249-56
pubmed: 26581029
Metab Eng. 2017 Jul;42:33-42
pubmed: 28550000
J Basic Microbiol. 2019 May;59(5):487-495
pubmed: 30768729
Microbiology (Reading). 2012 Nov;158(Pt 11):2724-2731
pubmed: 22956756
FEMS Microbiol Lett. 2016 Jan;363(1):fnv215
pubmed: 26564965
Appl Microbiol Biotechnol. 2018 Sep;102(18):8011-8021
pubmed: 29984395
Appl Microbiol Biotechnol. 2009 Feb;82(1):13-23
pubmed: 19082588
Metab Eng. 2007 Mar;9(2):217-27
pubmed: 17142079
mBio. 2018 Jun 12;9(3):
pubmed: 29895636
Appl Microbiol Biotechnol. 2007 Jan;73(5):1031-8
pubmed: 16941176
J Biol Chem. 2019 Jul 12;294(28):11023-11034
pubmed: 31160336
J Biol Chem. 2015 Oct 23;290(43):25920-32
pubmed: 26350459
J Ind Microbiol Biotechnol. 2006 Feb;33(2):94-104
pubmed: 16179985
Prog Mol Subcell Biol. 2013;54:39-63
pubmed: 24420710
Folia Microbiol (Praha). 1976;21(6):481-7
pubmed: 825418
J Bacteriol. 2008 Feb;190(4):1335-43
pubmed: 18083811
Nucleic Acids Res. 2013 Feb 1;41(3):1767-82
pubmed: 23248009
Trends Biotechnol. 2016 Mar;34(3):191-197
pubmed: 26702790
mBio. 2017 Jul 11;8(4):
pubmed: 28698272
Biotechnol J. 2017 Mar;12(3):
pubmed: 28105684
BMC Microbiol. 2014 Oct 25;14:265
pubmed: 25344463
Annu Rev Biochem. 2009;78:605-47
pubmed: 19344251
Antibiotics (Basel). 2019 Jun 30;8(3):
pubmed: 31262015
Mol Microbiol. 2005 Jun;56(5):1373-85
pubmed: 15882427
Appl Microbiol Biotechnol. 2012 Jul;95(1):61-75
pubmed: 22622839
Mol Microbiol. 2007 Jul;65(2):261-76
pubmed: 17630969
J Antibiot (Tokyo). 2017 May;70(5):534-541
pubmed: 28293039
Biochim Biophys Acta Gen Subj. 2017 Jan;1861(1 Pt A):2956-2970
pubmed: 27667172
BMC Microbiol. 2018 Jan 19;18(1):8
pubmed: 29351743
Microbiology (Reading). 2001 Jun;147(Pt 6):1525-1533
pubmed: 11390683
Antonie Van Leeuwenhoek. 2018 May;111(5):761-781
pubmed: 29605896
Sci Rep. 2017 Mar 15;7(1):200
pubmed: 28298624
Microbiology (Reading). 2008 Aug;154(Pt 8):2356-2370
pubmed: 18667568