New trends in the development of electrochemical biosensors for the quantification of microRNAs.
Amplification schemes
Biomarker
Electrochemical biosensor
miRNA
Journal
Journal of pharmaceutical and biomedical analysis
ISSN: 1873-264X
Titre abrégé: J Pharm Biomed Anal
Pays: England
ID NLM: 8309336
Informations de publication
Date de publication:
10 Sep 2020
10 Sep 2020
Historique:
received:
21
06
2020
revised:
13
07
2020
accepted:
14
07
2020
pubmed:
10
8
2020
medline:
22
6
2021
entrez:
10
8
2020
Statut:
ppublish
Résumé
MicroRNAs (miRNAs) are non-coding regulatory RNAs that play an important role in RNA silencing and post-transcriptional gene expression regulation. Since their dysregulation has been associated with Alzheimer disease, cardiovascular diseases and different types of cancer, among others, miRNAs can be used as biomarkers for early diagnosis and prognosis of these diseases. The methods commonly used to quantify miRNAs are, in general, complex, costly, with limited application for point-of-care devices or resource-limited facilities. Electrochemical biosensors, mainly those based on nanomaterials, have emerged as a promising alternative to the conventional miRNA detection methods and have paved the way to the development of sensitive, fast, and low-cost detection systems. This review is focused on the most relevant contributions performed in the field of electrochemical miRNAs biosensors between 2017 and the beginning of 2020. The main contribution of this article is the critical discussion of the different amplification strategies and the comparative analysis between amplified and non-amplified miRNA electrochemical biosensing and between the different amplification schemes. Particular emphasis was given to the importance of the nanostructures, enzymes, labelling molecules, and special sequences of nucleic acids or analogues on the organization of the different bioanalytical platforms, the transduction of the hybridization event and the generation the analytical signal.
Identifiants
pubmed: 32768875
pii: S0731-7085(20)31364-9
doi: 10.1016/j.jpba.2020.113478
pii:
doi:
Substances chimiques
MicroRNAs
0
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
113478Informations de copyright
Copyright © 2020. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of Competing Interest On behalf of the authors of the manuscript “New trends in the development of electrochemical biosensors for the quantification of microRNAs” by López Mujica, Gallay, Perrachione, Montemerlo, Tamborelli, Vaschetti, Reartes, Bollo, Rodríguez, Dalmasso, Rubianes and Rivas, I declare that there are no conflicts of interest.