Association of Glycolipids and Growth Factor Receptors in Lipid Rafts.
Density gradient
Ganglioside
Growth factor receptor
Lipid raft
Neural stem cell
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
10
8
2020
pubmed:
10
8
2020
medline:
18
3
2021
Statut:
ppublish
Résumé
The traditional methods to study lipid rafts and their association with membrane proteins are based mainly on the isolation of a detergent-resistant membrane by biochemical fractionation. However, the use of detergents may induce lipid segregation and/or redistribution of membrane proteins during the process of sample preparation. Here, we describe a detergent-free method to study the glycolipid and growth factor receptor interaction and their association with lipid rafts. This method combines the biochemical and immunoblotting tools with confocal microscopic imaging, which allows for evaluation and verification of the membrane protein interaction and association with the lipid rafts components in a multifaceted manner.
Identifiants
pubmed: 32770505
doi: 10.1007/978-1-0716-0814-2_8
pmc: PMC8460356
mid: NIHMS1733730
doi:
Substances chimiques
Detergents
0
Glycolipids
0
Membrane Lipids
0
Membrane Proteins
0
Receptors, Growth Factor
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
131-145Subventions
Organisme : NINDS NIH HHS
ID : R01 NS100839
Pays : United States
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